Recombinant Anti-H-FABP antibody [EPR6126] (ab133585)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6126] to H-FABP
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-H-FABP antibody [EPR6126]
See all H-FABP primary antibodies -
Description
Rabbit monoclonal [EPR6126] to H-FABP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human H-FABP aa 1-100. The exact sequence is proprietary.
-
Positive control
- Fetal muscle and fetal heart lysates; Human heart muscle tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 9.50 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6126 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).
|
Notes |
---|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa). |
Target
-
Function
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 2170 Human
- Entrez Gene: 14077 Mouse
- Entrez Gene: 79131 Rat
- Omim: 134651 Human
- SwissProt: P05413 Human
- SwissProt: P11404 Mouse
- SwissProt: P07483 Rat
- Unigene: 657242 Human
see all -
Alternative names
- 422 protein antibody
- Cardiac FABP antibody
- Cardiac Fatty Acid Binding Protein antibody
see all
Images
-
All lanes : Anti-H-FABP antibody [EPR6126] (ab133585) at 1/1000 dilution
Lane 1 : Fetal muscle tissue lysate
Lane 2 : Fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 15 kDa -
Immunohistochemical analysis of paraffin embedded Human heart tissue labelling H-FABP with ab133585 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab133585 has been referenced in 5 publications.
- Zhang X et al. Essential roles of exosome and circRNA_101093 on ferroptosis desensitization in lung adenocarcinoma. Cancer Commun (Lond) 42:287-313 (2022). PubMed: 35184419
- Che L et al. Valine increases milk fat synthesis in mammary gland of gilts through stimulating AKT/MTOR/SREBP1 pathway‡. Biol Reprod N/A:N/A (2019). PubMed: 30985894
- Vileigas DF et al. Landscape of heart proteome changes in a diet-induced obesity model. Sci Rep 9:18050 (2019). PubMed: 31792287
- Kühnel E et al. Placental-Specific Overexpression of sFlt-1 Alters Trophoblast Differentiation and Nutrient Transporter Expression in an IUGR Mouse Model. J Cell Biochem 118:1316-1329 (2017). PubMed: 27859593
- Nüsken E et al. Increased Rat Placental Fatty Acid, but Decreased Amino Acid and Glucose Transporters Potentially Modify Intrauterine Programming. J Cell Biochem 117:1594-603 (2016). PubMed: 26590355