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Products:Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Binding proteins
Overview
Product name
Anti-Cardiac FABP antibody
See all Cardiac FABP products (14) ...
Description
Rabbit polyclonal to Cardiac FABP
Specificity
ab45966 reacts with cardiac FABP, but does not react with liver or intestinal forms of this protein.
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Cow, Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Cardiac FABP.
(Peptide available as ab46185.)
Positive control
ab45966 gives a positive result in the following tissue lysates: Heart (Human) Tissue Lysate, Skeletal Muscle (Human) Tissue Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Mesenchymal Stem Cells >> Myogenesis
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Binding proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab45966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
IHC-P: 1/2000Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Function
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
Domain
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP05413
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- heart antibody
- Muscle fatty acid binding protein antibody
- 422 protein antibody
see all
Database links
- Entrez Gene: 281758 Cow
- Entrez Gene: 2170 Human
- Entrez Gene: 14077 Mouse
- Entrez Gene: 399532 Pig
- Omim: 134651 Human
- SwissProt: P10790 Cow
- SwissProt: P05413 Human
- SwissProt: P11404 Mouse
see all
Anti-Cardiac FABP antibody images:
Western blot - Cardiac FABP antibody (ab45966)
All lanes : Anti-Cardiac FABP antibody (ab45966) at 1 µg/ml
Lane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
Lane 2 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
Lane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)
Lane 4 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 15 kDa
Observed band size : 15 kDa
Ab45966 does not cross-react with small intestine or liver FABP.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cardiac FABP antibody (ab45966)
ab45966, at a 1/500 dilution, staining cardiac FABP in mouse heart by Immunohistochemistry, using formalin-fixed paraffin embedded tissue.
References for Anti-Cardiac FABP antibody (ab45966)
ab45966 has not yet been referenced specifically in any publications.
Publishing research using ab45966? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"