|
|
|
|
Western blot - Cardiac FABP antibody (ab45966)
(enlarge)
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cardiac FABP antibody (ab45966)
(enlarge)
|
|
|
|
|
Product Name
|
Cardiac FABP antibody
|
| |
See all Cardiac FABP antibodies (11)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to Cardiac FABP
|
|
Immunogen
|
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Cardiac FABP. (Peptide available as ab46185.)
|
|
Reacts with
|
Hu, Ms
|
|
Predicted to react
(species key)
|
Cow, Pig
(Due to sequence homology)
|
|
Specificity
|
ab45966 reacts with cardiac FABP, but does not react with liver or intestinal forms of this protein.
|
|
Tested applications
(see key)
|
IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IHC-P: 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
ab45966 gives a positive result in the following tissue lysates:
Heart (Human) Tissue Lysate,
Skeletal Muscle (Human) Tissue Lysate.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Stem Cells >> Mesenchymal Stem Cells >> Myogenesis
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Binding proteins
|
|
Relevance
|
Fatty Acid Binding Protein (FABP) is a small cytosolic protein responsible for the transport and deposition of fatty acids inside the cell. Several different isoforms of FABP are expressed in different tissue types. Cardiac isoform of FABP (cFABP) is expressed mainly in cardiac muscle tissue and in significantly lower concentration in skeletal muscles.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
Batch dependent within range: 0.90 - 0.90 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cardiac FABP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Cardiac FABP antibody images, references, products related to ab45966 and other tools.
|
| |
Cardiac FABP antibody images:
|
|
|
| |
 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cardiac FABP antibody (ab45966)
ab45966, at a 1/500 dilution, staining cardiac FABP in mouse heart by Immunohistochemistry, using formalin-fixed paraffin embedded tissue.
See Abreview
|
| |
| |
|
Search PubMed (MEDLINE) for references to Cardiac FABP
|
| |
Cardiac FABP antibody - more information
|
Customer reviews (feedback) regarding Cardiac FABP antibody |
Customer FAQs regarding Cardiac FABP antibody |
Protocols for Cardiac FABP antibody |
Price and availability of products related to Cardiac FABP antibody |
| |
Cardiac FABP antibody - related products:
|
|
|
| |
Cardiac FABP antibody - other tools:
|
Contact Abcam with a Technical Enquiry about Cardiac FABP antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
