Anti-Cardiac Troponin I antibody [284 (19C7)] (ab19615)

Catalog number(s): 19615

Product(s): Mouse monoclonal [284 (19C7)] to Cardiac Troponin I

Survey results
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Telephone section
Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint?
A. Yes
Q. Was it clear from the phone options which number you needed to press to get your issue resolved?
A. Yes

Protocol advice section
Q. Did Abcam's Scientific Support give you protocol advice?
A.Yes
Q. How did you feel about being given protocol advice?
A.I was not expecting to be given protocol advice, but was happy to receive this
Q. Did the product work successfully after following the advice?
A.No
Q. Why have you not tried the protocol advice? (Select all that apply.)
A.

Free of charge replacement section
Q. Did Abcam's Scientific Support give you a free of charge replacement?
A. Yes

Q. Did the replacement product work successfully?
A.Yes
Credit note / refund section
Q. Did Abcam's Scientific Support give you a credit note or a refund?
A.Yes

Q. Would you have preferred a different outcome from receiving a credit note or refund?
A.No
Overall satisfaction section
Q. How satisfied are you with how the complaint was handled?
A.Satisfied
Q. Could we have done anything to better resolve the problem?
A.Lot #GR50957-1 of ab19615 (cTnI antibody) did not work at all, even after contact with Abcam Tech Service. The replacement lot we received, #GR58712-1, worked but was weak compared to previous lots of this antibody that we've used in the past: #1078886, #978771, and #667212, which were all very strong. All lots tested in our lab were used under identical conditions, and it was noted that all were similar or near similar stock concentrations. We would have preferred a replacement vial from one of these stronger lots if it were possible, not from a "GR-" lot, which do not seem to work as well.

Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution?
A.No, I would not mind

Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products.
A.Thank you for sending me this survey and asking about my thoughts and problems.

ANSWER:

Thank you for your participation in Abcam'sfollow upsurvey.

If there is anything else I can do for you, or if you have questions to any of our products (e.g. primary antibodies, lysates, secondary antibodies, kits, controls, gels, proteins/peptides, slides, biochemicals, conjugation kits), please let me know.

I did check the available lots for ab19615 that we have in stock, but unfortunately the lot numbers you mentioned are not available anymore. I am sorry about that. Please let me know if I can assist you in any other way.

The most recent vial of antibody a19615 failed in IHC. Previous vials have worked well.

ANSWER:

I have issued a free of charge replacement with the order number ****, from a lot different than that of the vial that failed. The older lots that you mentioned are unfortunately sold out but I suspect that just the single vial was defective. We have not received any other complaints about the current lot or the one that failed for you.

To check the status of the order please contact our Customer Service team and reference this number.

Hi Tech,

Below is my customer problem and attached are the photos. Kindly provide me

with the solution.

Thanks.

Regrads,

Hi

Thank you for helping =)

We did it on a 24 wells plate.

Methods:

1. Media removed, cells rinsed twice with PBS 2. 400uL of ice-cold methanol

added, incubated for 6 minutes 3. Methanol removed, cells rinsed 3x with PBS

4. 400uL of 3% BSA in PBS added to all wells and removed after several

minutes 5. 250uL of Troponin antibody (1:50) added to sample wells 6. 250uL

of 3% BSA in PBS added to negative control wells 7. Cells incubated for an

hour, room temperature in dark 8. Cells rinsed 3x with PBS and twice with 3%

BSA in PBS 9. 250uL of secondary antibody added 10. Cells incubated for an

hour, room temperature in dark 11. Cells rinsed 3x with PBS 12. 200uL of

Hoescht added to all wells, incubated for 10 minutes, room temperature in

dark 13. Cells rinsed 3x with PBS 14. View cells under fluorescence

microscope

Attached are the pictures =)

Thank you very much.

ANSWER:

Thank you for taking the time to contact us with these details. I am sorry to hear the customer has had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality. Reviewing this case, I would like to offer some suggestions to help optimise the results from ab19615. I would also appreciate if you can confirm some further details: 1. Please provide the order number and date of purchase 2. Please confirm the antibody storage conditions 3. Are the cells allowed to dry after methanol removed and before rehydrating with the PBS? If the PBS is put on before the methanol has all evaporated, this can damage the cells. 4. I can recommend to try a different fixation. Try 4% PFA for 10 minutes only. You will need to include a different permeabilization step in this case, try 0.1% Triton for 10 minutes only. 5. As Cardiac Troponin I is a cytoplasmic protein, it is important to keep the cells permeabilised throughout the procedure. Try including 0.1% Tween 20 (a more gentle detergent that Triton) in the antibody dilution buffer, wash buffer and blocking buffer. 6. I can recommend to try incubating at 4oC overnight. Often provides more efficient and specific staining. 7. Please confirm if the secondary antibody working with other primary antibodies? Could you confirm if should detect the mouse IgG2b isotype? Please check and please provide the supplier and product code for the secondary antibody. In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

One our customers wants to purchase antibodies to test in sELISA and human samples.

They want to detect cardiac troponins T and I.

1) anti-cardiac troponin T : ab45932 to coat on surface, then the protein ab47005 and then the FITC conjugated antibody ab105439 for detection.

2) anti-cardiac troponin I : ab27972, then Cardiac Troponin I protein ab50803 and ab56357 that will be conjugated with FITC using the Easylink kit.

Would it work for their sELISA experiments?

ANSWER:

Thank you for contacting us.

I would recommend to use :

1) For the detection of Cardiac Troponin T :

- ab45932 as capture antibody (directed against amino acids 64-82) www.abcam.com/ab45932 ,

- ab9937 as protein target, www.abcam.com/ab9937 , (not ab47005 which is the immunogen peptide used to raise ab45932),

- ab105439 as detection antibody (directed against amino acids 64-82), www.abcam.com/ab105439 .

2) For the detection of Cardiac Troponin I :

- ab115704 as capture antibody, www.abcam.com/ab115704 ,

- ab50803 as protein target, www.abcam.com/ab50803 ,

- ab19615 as detection antibody (can be conjugated with FITC using the EasyLink conjugation kit), www.abcam.com/ab19615 .

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your helpful reply. Regarding Cardiac Troponin I protein ref. ab9936, I see that it has been only tested for WB. Do you have information using it for ELISA? In other case, do you have a specific one for ELISA?  Regards, 

ANSWER:

Thank you for your email.

The ab9936 product is a peptide which can be used to block antibodies ab7829, ab8290 and ab8291. This peptide can be used to block these antibodies irrespective of applications e.g. It is suitable to use with antibodies in Immunoprecipitation, western blot, Immunohistochemistry, ELSIA & also other blocking assays.

I hope this information will be helpful. Should you have any other question please do not hesitate to ask.