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Cardiac Troponin I protein (ab50803)

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Overview

  • Product nameCardiac Troponin I proteinSee all Cardiac Troponin I proteins and peptides ...
  • Protein descriptionRecombinant full length cardiac Troponin I protein (Human).
  • Expression hostE. coli

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Purification notesCombination of ammonium sulfate precipitation and ion exchange chromatography. Purity is greater than 90% by scanning SDS-PAGE.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5M Sodium chloride, 60mM Beta mercaptoethanol, 20mM Tris HCl, pH 7.5
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab50803 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    WB
  • Application notesSDS-PAGE: Use at an assay dependent dilution. MW ~28 kDa by SDS-PAGE.
    WB: Use at an assay dependent dilution.
    Calibrators and controls for immunoassays.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        cardiac muscleCardiac troponin ICardiomyopathy, familial hypertrophic, 7, included
        CMD1FFCMD2ACMH7cTnIFamilial hypertrophic cardiomyopathy 7MGC116817RCM1Tn1TniTNN I3TNNC 1TNNC1TNNI3TNNI3_HUMANTroponin ITroponin I cardiacTroponin I cardiac muscleTroponin I cardiac muscle isoformTroponin I type 3 cardiactroponin I, cardiac 3TroponinITtroponin I type 3 (cardiac)
      see all
  • FunctionTroponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Involvement in diseaseDefects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similaritiesBelongs to the troponin I family.

    • Target information above from: UniProt accession P19429 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for Cardiac Troponin I protein (ab50803)

    ab50803 has not yet been referenced specifically in any publications.

    Publishing research using ab50803 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab50803 Submit a Question for ab50803

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    stability of ab50803.

    I have been in contact with the lab regarding ab50803. They have confirmed that ab50803 and ab51319 are recombinant single chain cardiac troponin I-C polypeptides that are superior calibration and control materials for cardiac troponin I immunoassays. Read More

    Thank you for your questions.

    Actually we planned to use the kit in an assay for cardiac Troponin I and we had issues with the cTnI protein itself. So we have not tried the kit and unfortunately I can not give you any feedback concerning the kit ...

    Read More

    Thank you for contacting us.
    We have following anti Cardiac Troponin IgGs.
    http://www.abcam.com/cardiac-troponin-i-antibody-ab47003.html
    http://www.abcam.com/cardiac-troponin-i-antibody-284-19c7-ab19615.html
    http://www.abcam.com/cardiac-t...

    Read More

    ab50803: should have 70-72 ul, but has only 1 ul

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not contain the volumeas stated on the datasheet and for the inconvenien...

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    will test ab50803 in sELISA (as standard)

    Thank you for your phone call.

    DISCOUNT CODE: xxx
    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test ab50803 in ELISA. This code will give you: 1 freePROTEINbefore the expiration date. To r...

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    Custom conjugation of ab115704 to FITC.

    Thank you for calling Abcam yesterday.

    I have talked to our lab regarding the conjugation of ab115704 to FITC. Below are the details that they have provided:

    We could aim to supply 50ug of FITC labeled antibody in about 2 weeks, dependin...

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    One our customers wants to purchase antibodies to test in sELISA and human samples. They want to detect cardiac troponins T and I. 1) anti-cardiac troponin T : ab45932 to coat on surface, then the protein ab47005 and then the FITC conjugated antibo...

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    Thank you for contacting us. I would recommend to use : 1) For the detection of Cardiac Troponin T : - ab45932 as capture antibody (directed against amino acids 64-82) www.abcam.com/ab45932 , - ab9937 as protein target, www.abcam.com/...

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    Dear technical support: This customer raised an enquiry about ab50803 and ab50822. He would like to conduct the non-conventional application, and he wants to know which antigen (ab50803 and ab50822) can be used to pair with ab19615. Would you please h...

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    Thank you for your enquiry. I can confirm that ab50803 Cardiac Troponin I protein would be the most suitable for use with ab19615 Anti-Cardiac Troponin I antibody [284 (19C7)]. This is becuase it is full length recombinant human protein and should ...

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