Cardiac Troponin I protein (ab9936)

Dear Sir / Madam,
This order arrived yesterday for Cardiac Troponin I (Cat# ab9936). The vials were fully defrosted although still cold as they were sent to us in cold packs. There was no storage warning on the outside of the boxand onlythe insert sheet warns to store at -80 degrees cent. This is unfortunately a very important(and expensive) material for our workand it would be ideal to get the order replaced FOC if at all possible.
Kindest regards.

ANSWER:

Thank you very much for your call. As discussed I am happy to send you this confirmation:

Abcam takes product quality seriously and I would like to reassure you that we investigate all customer complaints.In addition to our in housequality control, we monitor customer feedback closely on a weekly basis and we have received no complaints about this product. Thus, we are not currently concerned about the general qualityof the Cardiac Troponin I protein ab9936 when shipped on this conditions.

We ship this protein in packaging with ice packs to provide extra temperature stability in transit. The ice pack may be thawed or even at room temperature when you receive it; please be assured that this is normal and that your product is safe to use in the tested and guaranteed applications (i.e. in Western blot/SDS-PAGE). Once you have received the vial, please follow the long-term storage instructions on the datasheet, i.e. storage at -80ºC and avoid freeze/thaw cycles. Also, the storage buffer including 8M urea should ensure suitable conditions for the protein to be used as stated on the datasheet.

Our Abpromise to you is that, should our product not work as stated on the datasheet, we will resolve the issue to your satisfaction, either with helpful advice to optimize your experiment or a replacement or refund of the product. Please contact our knowledgeable Scientific Support staff should you experience any issues while using our products. Contact information can be found on our website ('Contact us', or use mailto:technical@abcam.com).

I hope this information helps, but please don't hesitate to contact me for additional information or assistance.

I’ve set up the assay with ab9936, so I would need to try the ab50822. Could you provide a sample?

ANSWER:

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. This product is listed as "This peptide can be used with studies usinghttp://www.abcam.com/index.html?datasheet=7829,http://www.abcam.com/index.html?datasheet=8290andhttp://www.abcam.com/index.html?datasheet=8291.", all of which list ELISA as a tested application. If you are having difficulty using this in ELISA I would be happy to help. If you could send me your protocol and any data that you may have, I could look over that and if possible offer suggestions which may help. If we are unable to solve this problem I would be more than happy to send ab50222 as a replacement for ab9936.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

http://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Currently, I am using ab9936 for ELISA because you recommend it to me in the past. So, maybe it is better to store ab9936 in :   Preservative: 0.025% Sodium Azide Constituents: 50% Glycerol, 12.5µg/ml Aprotinin, 250mM Sodium chloride, 20mM Tris HCl, 2mM Magnesium chloride, 2mM Calcium chloride   Isn’t it?

ANSWER:

Thank you for your reply.

Further investigation into this protein revealed that this is very tightly bound and hydrophobic (http://www.rcsb.org/pdb/explore/explore.do?structureId=1MXL). As such the full peptide will need to be stored and used in a reduced form to be effective. Ab50822 is a single strand peptide and do not require these conditions.

While using this in ELISA is not ideal, the protein structure itself makes this a necessity. A shorter incubation at room temperature, one hour, with the sample may provide better results.

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Dear,   It is suitable for ELISA to store the protein ab9936 with the storage buffer that you recommend (8 M Urea, 50 mM Tris.HCl, 15 mM ß-mercaptoethanol, 1 mM EDTA (pH 8.0))? Thank you,

ANSWER:

Thank you for contacting Abcam.

I would not recommend using a product in ELISA with a storage buffer which contains8 M Urea, 15 mM ß-mercaptoethano as I would be wary of the buffer denaturing your antibody. I would recommend using a peptide which has a non-reducing buffer such as ab50822;http://www.abcam.com/cardiac-Troponin-I-protein-ab50822.html.

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

How is the purity of this protein determined?

ANSWER:

Thank you for contacting us.

The purity is determined by observation of a stained SDS-PAGE gel. The information I received from our source of the cardiac troponin protein is as follows:

Purity is estimated visually from the SDS PAGE gel. It probably exceeds 95%. Troponin-I is very susceptible to proteolysis and lower mwt bands on SDS PAGE represent TnI fragments (confirmed by western blot). The proteolysis occurs during the time the heart is in cold storage. With human hearts, it is extremely difficult to obtain human hearts that have been frozen immediately after death.

Please do not hesitate to contact us if you need any advice or more information.