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Cardiac Troponin I protein (ab9936)

Submit an Abreview Q&A (12)

Overview

  • Product nameCardiac Troponin I proteinSee all Cardiac Troponin I proteins and peptides ...
  • Expression hostNative

    • Properties

  • Purification notesSDS-PAGE - Major 24 kDa band (> 95%) Source:Human cardiac tissue Purified by Tn-C affinity chromatography
  • Biological activitySDS PAGE profile of a typical recombinant Troponin-I preparation (3.8ug) showed a major band at 24kDa and a minor band attributable to Troponin-I dimer. Please note: Troponin-I is very susceptible to proteolytic breakdown within cardiac tissue and it is difficult to isolate undegraded native human cardiac troponin-I. This product is greater than 70% intact and hence proteolytic fragments can be observed.
  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer8 M Urea, 50 mM Tris.HCl, 15 mM ß-mercaptoethanol, 1 mM EDTA (pH 8.0)
  • Concentration information loading...
  • Additional notesNative Human Cardiac Troponin-I
  • Research Areas

    • Applications

  • Application notesThis peptide can be used with studies using ab7829,ab8290,ab8291 and ab137719.

    • Protein info

    • Alternative names
        cardiac muscleCardiac troponin ICardiomyopathy, familial hypertrophic, 7, included
        CMD1FFCMD2ACMH7cTnIFamilial hypertrophic cardiomyopathy 7MGC116817RCM1Tn1TniTNN I3TNNC 1TNNC1TNNI3TNNI3TNNI3_HUMANTroponin ITroponin I cardiacTroponin I cardiac muscleTroponin I cardiac muscle isoformTroponin I type 3 cardiactroponin I, cardiac 3TroponinITtroponin I type 3 (cardiac)
      see all
  • FunctionTroponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Involvement in diseaseDefects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similaritiesBelongs to the troponin I family.

    • Target information above from: UniProt accession P19429 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Cardiac Troponin I protein images

    • Western blot - cardiac Troponin I protein (ab9936)
      Western blot - cardiac Troponin I protein (ab9936)
      Cardiac Troponin I protein (ab9936) at 2.7 µg (Coomassie blue staining of SDS gel)

      Performed under reducing conditions.

      Observed band size : 24 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 48 kDa (possible dimer).

    References for Cardiac Troponin I protein (ab9936)

    ab9936 has not yet been referenced specifically in any publications.

    Publishing research using ab9936 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab9936 Submit a Question for ab9936

    Displaying 1 - 10 of 12 results for Abreviews and Q&A

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    Do you suggest me using e.g. ab51319 Troponin I+C as a standard? What could be used as a control in that case? Do you have skeletal troponin I+C complex? I need a control with the same size and weight.

    I have been in contact with the lab who have confirmed that ab9936 is pure troponin-I and it does not contain Troponin-C. I would try and use this as the standard.
    ab9919 is pure skeletal muscle troponin-I (no troponin-C) and therefore would be good ...

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    Why do you have a high concentration of Urea at the buffer of ab9936 Cardiac Troponin I protein?

    Troponin-I can only be purified in urea containing buffers. In the absence of urea, at concentrations above ug/ml it precipitates and/or sticks to glass or plastic war. Even in the presence of 9 M urea it completely retains its functional ability to bind ...

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    Thank you for your questions.

    Actually we planned to use the kit in an assay for cardiac Troponin I and we had issues with the cTnI protein itself. So we have not tried the kit and unfortunately I can not give you any feedback concerning the kit ...

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    Thank you for contacting us.
    We have following anti Cardiac Troponin IgGs.
    http://www.abcam.com/cardiac-troponin-i-antibody-ab47003.html
    http://www.abcam.com/cardiac-troponin-i-antibody-284-19c7-ab19615.html
    http://www.abcam.com/cardiac-t...

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    Dear Sir / Madam,
    This order arrived yesterday for Cardiac Troponin I (Cat# ab9936). The vials were fully defrosted although still cold as they were sent to us in cold packs. There was no storage warning on the outside of the boxand onlythe insert sh...

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    Thank you very much for your call. As discussed I am happy to send you this confirmation:

    Abcam takes product quality seriously and I would like to reassure you that we investigate all customer complaints.In addition to our in housequality contr...

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    I’ve set up the assay with ab9936, so I would need to try the ab50822. Could you provide a sample?

    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guara...

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    Currently, I am using ab9936 for ELISA because you recommend it to me in the past. So, maybe it is better to store ab9936 in :   Preservative: 0.025% Sodium Azide Constituents: 50% Glycerol, 12.5µg/ml Aprotinin, 250mM Sodium chloride, 20mM Tris HCl, 2m...

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    Thank you for your reply.

    Further investigation into this protein revealed that this is very tightly bound and hydrophobic (http://www.rcsb.org/pdb/explore/explore.do?structureId=1MXL). As such the full peptide will need to be stored and used i...

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    Dear,   It is suitable for ELISA to store the protein ab9936 with the storage buffer that you recommend (8 M Urea, 50 mM Tris.HCl, 15 mM ß-mercaptoethanol, 1 mM EDTA (pH 8.0))? Thank you,

    Thank you for contacting Abcam.

    I would not recommend using a product in ELISA with a storage buffer which contains8 M Urea, 15 mM ß-mercaptoethano as I would be wary of the buffer denaturing your antibody. I would recommend using a pepti...

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    How is the purity of this protein determined?

    Thank you for contacting us.

    The purity is determined by observation of a stained SDS-PAGE gel. The information I received from our source of the cardiac troponin protein is as follows:

    Purity is estimated visually from the SDS PAGE ...

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    I need to use this protein in cell culture, and there was concern about the purity of this protein and the endotoxin level. Is there anything we can do to further purify the protein? Do you offer it in a form that is compatible with culture?

    Thank you for your inquiry. I heard back from the supplying lab that this protein is purified using ion-exchange and two rounds of calcium-dependent troponin-C affinity chromatography in 8M urea solution. Therefore, little else binds to the TnC col...

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    Thank you for your helpful reply. Regarding Cardiac Troponin I protein ref. ab9936, I see that it has been only tested for WB. Do you have information using it for ELISA? In other case, do you have a specific one for ELISA?  Regards, 

    Thank you for your email. The ab9936 product is a peptide which can be used to block antibodies ab7829, ab8290 and ab8291. This peptide can be used to block these antibodies irrespective of applications e.g. It is suitable to use with antibodies in...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"