Overview

  • Product nameAnti-Cardiac Troponin T antibodySee all Cardiac Troponin T primary antibodies ...
  • Description
    Rabbit polyclonal to Cardiac Troponin T
  • Tested applicationsIHC-P, Sandwich ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Rabbit, Guinea pig, Cow, Cat, Dog

    Does not react with

    Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human cardiac Troponin T.

    (Peptide available as ab47005.)

  • Positive control
    • This antibody gave a positive signal in Human Fetal Heart Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab45932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) as Capture.
ICC/IF 1/400. Incubate for 1-2 hours.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).Can be blocked with Human Cardiac Troponin T peptide (ab47005).

Target

  • FunctionTroponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificityHeart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • Involvement in diseaseDefects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • Sequence similaritiesBelongs to the troponin T family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTC antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 antibody
    • Troponin T2 cardiac antibody
    see all

Anti-Cardiac Troponin T antibody images

  • Standard Curve for Cardiac Troponin T (Analyte: Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5ug/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5ug/ml.
  • Anti-Cardiac Troponin T antibody (ab45932) at 1 µg/ml + Heart (Human) Whole Cell Lysate - fetal normal tissue at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • ab45932 staining cardiac Troponin T in human fetal cardiomyocytes by ICC/IF. The cells were paraformaldehyde fixed, permeabilized in 0.1% (PBS) saponin buffer and blocked in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 (0.1% saponin, 10% goat serum, 0.01% triton) and incubated with sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (L+H), diluted 1/600 was used as secondary.

    See Abreview

  • IHC image of Cardiac Troponin T staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45932, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ab45932 staining Cardiac Troponin T in rat cardiac cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in 3% paraformaldehyde. Samples were incubated with primary antibody (1/400 in diluent) for 1 hour at room temperature. A Cy3®-conjugated goat anti-rabbit polyclonal IgG (1/300) was used as the secondary antibody.

    See Abreview

Protocols

References for Anti-Cardiac Troponin T antibody (ab45932)

This product has been referenced in:
  • Hassiotou F  et al. Expression of the Pluripotency Transcription Factor OCT4 in the Normal and Aberrant Mammary Gland. Front Oncol 3:79 (2013). ICC/IF ; Human . Read more (PubMed: 23596564) »
  • Gentile A  et al. Human epicardium-derived cells fuse with high efficiency with skeletal myotubes and differentiate toward the skeletal muscle phenotype: a comparison study with stromal and endothelial cells. Mol Biol Cell 22:581-92 (2011). ICC/IF ; Human . Read more (PubMed: 21209317) »

See all 3 Publications for this product

Product Wall

Thank you for your enquiry. We are not aware of any references for the antibody ab45932 that include western blot data demonstrating recognition of intact and fragmented troponin T. There is a western blot of human heart tissue on the datasheet which s...

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Thank you for contacting us.

The antibody ab45932 recognizes the amino acid sequence of the immunogen, so it should be capable of detecting protein fragments that contain this sequence, using the applications listed on the datasheet: western...

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Application Immunocytochemistry/ Immunofluorescence
Sample Rabbit Cell (RAT cardiac cells)
Specification RAT cardiac cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton x-100 in 3% paraformaldehyde
Username

Tova Volberg

Verified customer

Submitted Jul 17 2012

Thank you for your inquiry.

I am assuming your customer wrote an Abreview for ab45932 and rabbit?

If so, I can confirm that we received the Abreview (today 17.07.2012)and one of my colleagues is working on it.

Since the co...

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Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (neonatal cardiomyocyte)
Specification neonatal cardiomyocyte
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Mrs. Jamie Soto

Verified customer

Submitted Jun 15 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Kardiomyocyten)
Specification Kardiomyocyten
Fixative CytoPerm/Fix (BD)
Permeabilization Yes - WashPerm (BD)
Blocking step WashPerm (BD) as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted May 07 2012

DISCOUNT CODE: *******
Expiration date: DD MM YYYY
I am very pleased to hear you would like to accept our offer and test ab45932 in Rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please...

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Thank you for calling Abcam earlier today.

Please let me know if the protocol tips we discussed prove successful or not.

The antibody is covered under our Abpromise for six months and is guaranteed to work in ICC/IF on human samples...

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Thank you for contacting us. I would recommend to use : 1) For the detection of Cardiac Troponin T : - ab45932 as capture antibody (directed against amino acids 64-82) www.abcam.com/ab45932 , - ab9937 as protein target, www.abcam.c...

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (fetal cardiomyocytes)
Specification fetal cardiomyocytes
Fixative Paraformaldehyde
Permeabilization Yes - saponin 0.1% in PBS
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 37°C
Username

Dr. Silvia Martin-Puig

Verified customer

Submitted Dec 16 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"