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Drosophila third instar larvae were dissected and fixed to get a fillet preparation. Samples were fixed in formaldehyde, permeabilized using Triton X-100, blocked with 5% serum for 1 hour at 25°C and then incubated with ab58736 at a 1/500 dilution. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.Cardioactive Peptide shows positive staining colocalizing with neuronal marker for HRP.
Image courtesy of an anonymous Abreview.
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