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Anti-Cardioactive Peptide antibody (ab58736)

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Overview

Product name

Anti-Cardioactive Peptide antibody

Description

Rabbit polyclonal to Cardioactive Peptide

Tested applications

WB, ELISA, ICC/IFmore details

Cross reactivity

Reacts with

Recombinant Fragment

Predicted to work with

Fruit fly (Drosophila melanogaster)

Immunogen

Synthetic peptide corresponding to N-terminal residues of Drosophila melanogaster Cardioactive Peptide

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • - Anti-Cardioactive Peptide antibody (ab58736)- Anti-Cardioactive Peptide antibody (ab58736) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab58736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 µg/mlPredicted molecular weight: 18 kDa.(for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.)

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    ELISA

     ELISA: Use at an assay d...Read more →

    ELISA: Use at an assay dependent dilution.

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    ICC/IF

    Five stars (1 Abreview) ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent concentration.

Target

Relevance

Cardioactive peptide (CCAP) is a cardioregulatory neurohormone that increases heart beat rate during adult wing inflation. This protein has no effect on beat amplitude. The effect of cardioactive peptide is both ino- and conotrophic. Cardioactive peptide has tissue specificity within the central nervous system and most neurons exhibit coexpression with burs.

Cellular localization

Secreted

Alternative names

  • Cardioacceleratory peptide 2a antibody
  • CCAP antibody
  • CG4910 antibody
  • Crustacean cardioactive peptide antibody
  • DmCCAP antibody
see all

Database links

Anti-Cardioactive Peptide antibody images:

  - Anti-Cardioactive Peptide antibody (ab58736)

- Anti-Cardioactive Peptide antibody (ab58736)

Drosophila third instar larvae were dissected and fixed to get a fillet preparation. Samples were fixed in formaldehyde, permeabilized using Triton X-100, blocked with 5% serum for 1 hour at 25°C and then incubated with ab58736 at a 1/500 dilution. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.

Cardioactive Peptide shows positive staining colocalizing with neuronal marker for HRP.

Image courtesy of an anonymous Abreview.

See Abreview

References for Anti-Cardioactive Peptide antibody (ab58736)

ab58736 has not yet been referenced specifically in any publications.

Publishing research using ab58736? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"