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Cardiotrophin 1 (CTF1) Human ELISA Kit (ab99996)

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Overview

  • Product nameCardiotrophin 1 (CTF1) Human ELISA KitSee all Cardiotrophin 1 kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 20 pg/ml
  • Range
    16.46 pg/ml - 12000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.2 76% - 112%
    Serum 132.4 120% - 142%
    Plasma 101.2 70% - 134%

  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam's Cardiotrophin 1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Cardiotrophin 1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Cardiotrophin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Cardiotrophin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Cardiotrophin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Cardiotrophin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionInduces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.
  • Tissue specificityHighly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession Q16619 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Cardiophin 1Cardiophin1Cardiotrophin I
        Cardiotrophin-1CardiotrophinICT 1CT-1CT1 CTF 1Ctf1CTF1_HUMAN
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab99996 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Cardiotrophin 1 (CTF1) Human ELISA Kit images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab99996.

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab99996.

      • ELISA - Cardiotrophin 1 Human ELISA Kit (ab99996)
        ELISA - Cardiotrophin 1 Human ELISA Kit (ab99996)

      Protocols

      References for Cardiotrophin 1 (CTF1) Human ELISA Kit (ab99996)

      ab99996 has not yet been referenced specifically in any publications.

      Publishing research using ab99996 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab99996 Submit a Question for ab99996

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      Les escribo para realizar una consulta sobre el kit de ELISA para CT1 humana (ab99996). Me interesa saber si este kit funciona bien conmuestras obtenidas de homogeneizados de tejidos. Tambien necesito saber si se ha descrito interaccion con CT1 de rata y ...

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      Thank you for contacting us. Sorry to reply to in English but my Spanish colleague is currently out of the office and my Spanish is unfortunately not good enough.

      The source of theCardiotrophin 1 Human ELISA Kit (ab99996)informed meit isvery acco...

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      Thank you for your help in trouble shooting for Cardiotrophin 1 Human ELISA (Cat #: ab99996).  Please find assay result attached. 

      Thank you for your inquiry. After reviewing your standard data, I must say the curve actually looks good (see linear and log-log plots in attachments).  As you can see, the signal strength is strong, the linearity is very nice (≥0.98), the duplicates a...

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      Recently, we bought Human Cardiotrophin-1 ELISA kit. Abcam kit does not recover very well calibrators when they are used as samples which assess the recovery performance of assay and also our samples are not detectable with the Abcam as they read lower th...

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      Thank you very much for your patience. I've finally heard back from the senior scientist, and he has the following response (I've placed it in quotes): "To be honest, I really don’t see an issue with the standard curve as it is giving strong signal re...

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