Recombinant
RabMAb

Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)

Overview

  • Product name
    Anti-Casein Kinase 1 alpha antibody [EPR1961(2)]
    See all Casein Kinase 1 alpha primary antibodies
  • Description
    Rabbit monoclonal [EPR1961(2)] to Casein Kinase 1 alpha
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Casein Kinase 1 alpha aa 250-350.

  • Positive control
    • MCF-7, HeLa, Jurkat, Rat brain lysates, RAW 264.7, PC-12 and HT-1376 cell lysates
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 39 kDa).

For unpurified use at 1/1000 - 1/10000

Target

  • Function
    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • Casein kinase 1 alpha 1 antibody
    • Casein kinase I isoform alpha antibody
    • CK1 antibody
    • CK1A antibody
    • CKI alpha antibody
    • CKI-alpha antibody
    • CKIa antibody
    • Clock regulator kinase antibody
    • Csnk1a1 antibody
    • Down regulated in lung cancer antibody
    • HLCDGP1 antibody
    • KC1A_HUMAN antibody
    • PRO2975 antibody
    see all

Images

  • All lanes : Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296) at 1/1000 dilution (purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
    Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 39 kDa

    Blocking and diluting buffer: 5% NFDM/TBST

  • Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296) at 1/2000 dilution + RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 39 kDa

    Blocking and diluting buffer: 5% NFDM/TBST

  • All lanes : Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296) at 1/1000 dilution (purified)

    Lane 1 : Rat brain lysates
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 39 kDa

    Blocking and diluting buffer: 5% NFDM/TBST

  • All lanes : Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296) at 1/1000 dilution (unpurified)

    Lane 1 : MCF7 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HT-1376 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 39 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse macrophage)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
15 µg
Specification
Mouse macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 20 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (lymphoid cell)
Gel Running Conditions
Reduced Denaturing (10 % gel)
Loading amount
10 µg
Specification
lymphoid cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 18 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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