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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab85320? Please let us know so that we can cite the reference in this datasheet
ab85320 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - Casein Kinase 1 delta antibody [AF12G4] (ab85320)](/ps/datasheet/images/85/ab85320/Casein-Kinase-1-delta-Primary-antibodies-ab85320-1.jpg)
Western blot - Casein Kinase 1 delta antibody [AF12G4] (ab85320)
All lanes : Anti-Casein Kinase 1 delta antibody [AF12G4] (ab85320) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : SK-N-MC cell lysate
Lane 4 : 293T cell lysate
Lane 5 : L929 cell lysate
Predicted band size : 47 kDa
Observed band size : 47 kDa
](/ps/datasheet/images/85/ab85320/Casein-Kinase-1-delta-Primary-antibodies-ab85320-2.jpg)
Flow Cytometry-Anti-Casein Kinase 1 delta antibody [AF12G4](ab85320)
Overlay histogram showing HeLa cells stained with ab85320 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85320, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 1 delta antibody [AF12G4] (ab85320)](/ps/datasheet/images/85/ab85320/Casein-Kinase-1-delta-Primary-antibodies-ab85320-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 1 delta antibody [AF12G4] (ab85320)
IHC image of ab85320 staining in human Alzheimer brain (hippocampus) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85320, 0.2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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