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Anti-Casein Kinase 1 delta antibody [AF12G4] (ab85320)

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Overview

Product name

Anti-Casein Kinase 1 delta antibody [AF12G4]
See all Casein Kinase 1 delta products (5) ...

Description

Mouse monoclonal [AF12G4] to Casein Kinase 1 delta

Tested applications

Flow Cyt, WB, IP, ELISAmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

His tagged recombinant Human Casein Kinase 1 delta protein, purified from E.coli.

Positive control

HeLa, K562, SK-N-MC, 293T and L929 cell lysates.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.03% Sodium Azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride

Concentration

Concentration information loading...

Purity

Ammonium Sulphate Precipitation

Clonality

Monoclonal

Clone number

AF12G4

Isotype

IgG2b

Light chain type

kappa

Research areas

Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Tangles & Tau
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53

  • Western blot - Casein Kinase 1 delta antibody [AF12G4] (ab85320)Western blot - Casein Kinase 1 delta antibody [AF12G4] (ab85320) image (enlarge)

  • Flow Cytometry-Anti-Casein Kinase 1 delta antibody [AF12G4](ab85320)Flow Cytometry-Anti-Casein Kinase 1 delta antibody [AF12G4](ab85320) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab85320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm.

Tissue specificity

Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. In blood, highly expressed in hemopoietic cells and mature granulocytes. Also found in monocytes and lymphocytes.

Involvement in disease

Defects in CSNK1D are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.

Sequence similarities

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain.

Post-translational
modifications

Autophosphorylated on serine and threonine residues.

Cellular localization

Cytoplasm. Nucleus.

Target information above from: UniProt accessionP48730 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Casein kinase I delta isoform antibody
  • Casein kinase I isoform delta antibody
  • CKI delta antibody
  • CKI-delta antibody
  • CKId antibody
  • CSNK 1D antibody
  • CSNK1D antibody
  • HCKID antibody
  • KC1D_HUMAN antibody
  • Protein kinase CK1 delta antibody
see all

Database links

see all

Anti-Casein Kinase 1 delta antibody [AF12G4] images:

  Western blot - Casein Kinase 1 delta antibody [AF12G4] (ab85320)

Western blot - Casein Kinase 1 delta antibody [AF12G4] (ab85320)

All lanes : Anti-Casein Kinase 1 delta antibody [AF12G4] (ab85320) at 1/5000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : SK-N-MC cell lysate
Lane 4 : 293T cell lysate
Lane 5 : L929 cell lysate


Predicted band size : 47 kDa
Observed band size : 47 kDa

  Flow Cytometry-Anti-Casein Kinase 1 delta antibody [AF12G4](ab85320)

Flow Cytometry-Anti-Casein Kinase 1 delta antibody [AF12G4](ab85320)

Overlay histogram showing HeLa cells stained with ab85320 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85320, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Casein Kinase 1 delta antibody [AF12G4] (ab85320)

ab85320 has not yet been referenced specifically in any publications.

Publishing research using ab85320? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"