Anti-Casein Kinase 1 delta/CSNK1D antibody (ab151793)
Key features and details
- Rabbit polyclonal to Casein Kinase 1 delta/CSNK1D
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Casein Kinase 1 delta/CSNK1D antibody
See all Casein Kinase 1 delta/CSNK1D primary antibodies -
Description
Rabbit polyclonal to Casein Kinase 1 delta/CSNK1D -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human Casein Kinase 1 delta/CSNK1D aa 144-361.
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Positive control
- HeLa and BCL-1 whole cell lysates; HeLa cells; Human hepatoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. -
Tissue specificity
Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. In blood, highly expressed in hemopoietic cells and mature granulocytes. Also found in monocytes and lymphocytes. -
Involvement in disease
Defects in CSNK1D are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms. -
Sequence similarities
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated on serine and threonine residues. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 407122 Cow
- Entrez Gene: 1453 Human
- Entrez Gene: 104318 Mouse
- Entrez Gene: 64462 Rat
- Omim: 600864 Human
- SwissProt: P35508 Cow
- SwissProt: P48730 Human
- SwissProt: Q9DC28 Mouse
see all -
Alternative names
- Casein Kinase 1 delta antibody
- Casein kinase I delta isoform antibody
- Casein kinase I isoform delta antibody
see all
Images
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Anti-Casein Kinase 1 delta/CSNK1D antibody (ab151793) at 1/2000 dilution + BCL-1 whole cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS PAGE -
Anti-Casein Kinase 1 delta/CSNK1D antibody (ab151793) at 1/2000 dilution + HeLa cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS PAGE -
Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling Casein Kinase 1 delta/CSNK1D with ab151793 at 1/500 dilution (green). Alpha-tubulin filaments were also labeled (Red).
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Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue, labeling Casein Kinase 1 delta/CSNK1D with ab151793 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab151793 has not yet been referenced specifically in any publications.