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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
Overview
Product name
Anti-Casein Kinase 1 delta antibody
See all Casein Kinase 1 delta products (5) ...
Description
Rabbit polyclonal to Casein Kinase 1 delta
Specificity
ab37971 recognizes Casein Kinase 1 delta.
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
KLH conjugated synthetic peptide, derived from C terminal within residues 336-352 of Casein Kinase 1 delta (Human).
Positive control
HeLa cell lysate (WB), human breast carcinoma and human hepatocarcinoma tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Eluted out with both high and low pH buffers and neutralized immediately after elution, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Tangles & Tau
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/500.Detects a band of approximately 47 kDa.
ELISA
ELISA: 1/1000ELISA: 1/1000
IHC-P: 1/50 - 1/100.
Target
Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm.
Tissue specificity
Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. In blood, highly expressed in hemopoietic cells and mature granulocytes. Also found in monocytes and lymphocytes.
Involvement in disease
Defects in CSNK1D are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.
Sequence similarities
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated on serine and threonine residues.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionP48730
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Casein kinase I delta isoform antibody
- Casein kinase I delta isoform antibody
- Casein kinase I isoform delta antibody
see all
Database links
- Entrez Gene: 1453 Human
- Omim: 600864 Human
- SwissProt: P48730 Human
- Unigene: 631725 Human
Anti-Casein Kinase 1 delta antibody images:
Western blot - Casein Kinase 1 delta antibody (ab37971)
Anti-Casein Kinase 1 delta antibody (ab37971) + HeLa cell lysate
Secondary
HRP-anti-rabbit
Observed band size : 47 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Casein Kinase 1 delta antibody (ab37971)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with ab37971, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. HC = hepatocarcinoma.
References for Anti-Casein Kinase 1 delta antibody (ab37971)
ab37971 has not yet been referenced specifically in any publications.
Publishing research using ab37971? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"