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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
Overview
Product name
Anti-Casein Kinase 1 delta antibody
See all Casein Kinase 1 delta products (5) ...
Description
Goat polyclonal to Casein Kinase 1 delta
Specificity
This antibody is expected to recognise both reported isoforms of Casein Kinase 1 delta
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Chimpanzee, Macaque Monkey
Immunogen
Synthetic peptide: DREERLRHSRNPATR from the internal region of the protein (Human)
DREERLRHSR NPATR
Positive control
HEK 293 cell lysate and lysates of Human Brain
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Tangles & Tau
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
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Western blot - Casein Kinase 1 delta antibody (ab48031)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab48031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
Application notes
ELISA: Use at an assay dependent dilution.
Antibody detection limit dilution 1/64,000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm.
Tissue specificity
Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. In blood, highly expressed in hemopoietic cells and mature granulocytes. Also found in monocytes and lymphocytes.
Involvement in disease
Defects in CSNK1D are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.
Sequence similarities
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated on serine and threonine residues.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionP48730
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Casein kinase I delta isoform antibody
- Casein kinase I isoform delta antibody
- CKI delta antibody
see all
Database links
- Entrez Gene: 1453 Human
- Entrez Gene: 104318 Mouse
- Entrez Gene: 64462 Rat
- Omim: 600864 Human
- SwissProt: P48730 Human
- SwissProt: Q9DC28 Mouse
- SwissProt: Q06486 Rat
- Unigene: 631725 Human
see all
Anti-Casein Kinase 1 delta antibody images:
Western blot - Casein Kinase 1 delta antibody (ab48031)
Anti-Casein Kinase 1 delta antibody (ab48031) at 0.3 µg/ml + HEK 293 cell lysate in RIPA buffer at 35 µg
Predicted band size : 47 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
References for Anti-Casein Kinase 1 delta antibody (ab48031)
This product has been referenced in:
- Zyss Det al. Casein kinase I delta controls centrosome positioning during T cell activation. J Cell Biol 195:781-97 (2011).Read more (PubMed: 22123863) »
See 1 publication for this product
Publishing research using ab48031? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"