Anti-Casein Kinase 1 gamma 2 antibody (ab64829)
Overview
- Product nameAnti-Casein Kinase 1 gamma 2 antibodySee all Casein Kinase 1 gamma 2 primary antibodies ...
- DescriptionRabbit polyclonal to Casein Kinase 1 gamma 2
- Tested applicationsELISA, WB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat - Immunogen
Synthetic peptide derived from N-terminus of human Casein Kinase 1 gamma 2.
- Positive controlHeLa and 293 cell extracts.
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab64829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: 1/10000. |
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
Target
- RelevanceCasein kinase 1 gamma 2 (CSNK1G2) is a serine/threonine protein kinase which shows preferential utilization of acidic proteins such as caseins as a substrate. It can phosphorylate a large number of proteins.
- Cellular localizationCytoplasmic
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Database links
- Entrez Gene: 1455 Human
- Entrez Gene: 1455 Human
- Entrez Gene: 1455 Human
- Entrez Gene: 103236 Mouse
- Entrez Gene: 65278 Rat
- Omim: 602214 Human
- SwissProt: P78368 Human
- SwissProt: P78368 Human
- SwissProt: P78368 Human
- SwissProt: Q8BVP5 Mouse
- SwissProt: Q62762 Rat
- Unigene: 651905 Human
see all
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Alternative names
- Casein kinase 1, gamma 2 antibodyCasein kinase I isoform gamma 2 antibodyCK1g2 antibody
- CKI gamma 2 antibodyCSNK1G 2 antibodyCSNK1G2 antibody
see all
Anti-Casein Kinase 1 gamma 2 antibody images
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Western blot - Anti-Casein Kinase 1 gamma 2 antibody (ab64829)This image is courtesy of an anonymous AbreviewLanes 1 - 2 : Anti-Casein Kinase 1 gamma 2 antibody (ab102554)
Lanes 3 - 4 : Anti-Casein Kinase 1 gamma 2 antibody - C-terminal (ab70862)
Lanes 5 - 6 : Anti-Casein Kinase 1 gamma 2 antibody (ab64829) at 1/500 dilution
Lane 1 : U2OS cells expressing FLAG-tagged CK1G2 plasmid
Lane 2 : U2OS cells expressing FLAG tag only plasmid
Lane 3 : U2OS cells expressing FLAG-tagged CK1G2 plasmid
Lane 4 : U2OS cells expressing FLAG only
Lane 5 : U2OS cells expressing FLAG-tagged CK1G2 plasmid
Lane 6 : U2OS cells expressing FLAG only
Secondary
Lanes 5 - 6 : HRP-conjugated Goat anti-rabbit polyclonal at 1/1000 dilution
developed using the ECL technique
Predicted band size : 47 kDaThis image is courtesy of an anonymous Abreview
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Western blot - Casein Kinase 1 gamma 2 antibody (ab64829)All lanes : Anti-Casein Kinase 1 gamma 2 antibody (ab64829) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : 293 cell extract
Lane 3 : 293 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 47 kDa
Observed band size : 47 kDa
Additional bands at : 25 kDa,34 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Casein Kinase 1 gamma 2 antibody (ab64829)
This product has been referenced in:
- Del Valle-Pérez B et al. Coordinated Action of CK1 Isoforms in Canonical Wnt Signaling. Mol Cell Biol 31:2877-88 (2011). WB ; Human . Read more (PubMed: 21606194) »
- Casagolda D et al. A p120-catenin-CK1epsilon complex regulates Wnt signaling. J Cell Sci 123:2621-31 (2010). WB ; Human . Read more (PubMed: 20940130) »
