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Product Name
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Caspase 1 Fluorometric Assay Kit
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Product type
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Kits
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Description
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Caspase 1 Fluorometric Assay Kit
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Kits/ Lysates/ Other >> Kits >> Apoptosis Kits >> Caspase Assays Kits
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
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Components
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| Kit Components | Volume |
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| Cell Lysis Buffer | 100ml | | 2X Reaction Buffer | 4 X 2ml | | YVAD-AFC(1mM) | 0.5ml | | DTT(1M) | 0.4ml |
Note: The kit provides approx. 100 assays.
For a detailed set of instructions please see the protocol.
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Storage instructions
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Store kit at -20°C (Store Lysis Buffer and 2X Reaction Buffer at +4°C after opening)
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Notes
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The Caspase 1 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD.
The assay is based on detection of cleavage of substrate YVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). YVAD-AFC emits blue light (wavelenght max = 400 nm); upon cleavage of the substrate by caspase-1 or related caspases, free AFC emits a yellow-green fluorescence ( Wavelength max = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.
Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase 1 activity.
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Material safety datasheet (MSDS) for this product:
Caspase 1 Fluorometric Assay Kit MSDS
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Relevance
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Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase 1 is similar to the cell death gene CED3 of C. elegans and regulates multiple proinflammatory cytokines, including Interleukin 1b and interferon-gamma-inducing factor. Caspase 1 plays a role in down stream of Caspase 8 which is involved in Fas-mediated apoptosis.
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Caspase 1 Fluorometric Assay Kit (ab39412) |
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Caspase 1 Fluorometric Assay Kit is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Caspase 1
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Caspase 1 Fluorometric Assay Kit - more information
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Customer reviews (feedback) regarding Caspase 1 Fluorometric Assay Kit |
Customer FAQs regarding Caspase 1 Fluorometric Assay Kit |
Protocols for Caspase 1 Fluorometric Assay Kit |
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Contact Abcam with a Technical Enquiry about Caspase 1 Fluorometric Assay Kit |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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