![Western blot - Caspase 1 antibody [14F468] (ab14367) image](#ab14367.jpg)
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Product name
Anti-Caspase 1 antibody [14F468]
See all Caspase 1 products (17) ...
Description
Mouse monoclonal [14F468] to Caspase 1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Rat, Pig
Immunogen
Synthetic peptide: RKVRFSFEQPDGRAQMPTTE , corresponding to amino acids 371-390 of Human Caspase 1.
RKVRFSFEQPDGRAQMPTTE
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
14F468
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
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Western blot - Caspase 1 antibody [14F468] (ab14367)
![Western blot - Caspase 1 antibody [14F468] (ab14367) image](/ps/datasheet/Images/14/ab14367/ab14367.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab14367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 45 kDa.
Apoptosis kit available (ab39412.)
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
Tissue specificity
Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain.
Post-translational
modifications
The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP29466
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CASP 1 antibody
- CASP-1 antibody
- CASP1 antibody
see all
Database links
- Entrez Gene: 834 Human
- Entrez Gene: 397319 Pig
- Entrez Gene: 25166 Rat
- Omim: 147678 Human
- SwissProt: P29466 Human
- SwissProt: Q9N2I1 Pig
- SwissProt: P43527 Rat
- Unigene: 2490 Human
see all
Anti-Caspase 1 antibody [14F468] images:
Western blot - Caspase 1 antibody [14F468] (ab14367)
Western blot analysis of Caspase 1 in HeLa cell lysate using ab14367 at 0.5 ug/ml.
References for Anti-Caspase 1 antibody [14F468] (ab14367)
ab14367 has not yet been referenced specifically in any publications.
Publishing research using ab14367? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"