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Caspase 1 protein (Active) (ab39901)

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Overview

  • Product nameCaspase 1 protein (Active)See all Caspase 1 proteins and peptides ...
  • Protein descriptionRecombinant full length protein (Human)
  • Expression hostE. coli

    • Properties

  • Biological activityActivity : >5000 units/mg
  • FormLyophilised:Reconstitute to 1 unit per µl in PBS containing 15% glycerol
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Concentration information loading...
  • Additional notesActive caspase 1 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. The active caspase 1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA)
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab39901 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution. We recommend using 1 unit/assay for analyzing caspase activity.

    Protein info

    • Alternative names
        CASP1Caspase 1 apoptosis related cysteine peptidaseICE
        IL1 B convertaseIL1 beta converting enzymeIL1BCIL1BCEInterleukin 1 B converting enzymeInterleukin 1 beta convertaseP45
      see all
  • RelevanceCaspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase 1 is similar to the cell death gene CED3 of C. elegans and regulates multiple proinflammatory cytokines, including Interleukin 1b and interferon-gamma-inducing factor. Caspase 1 plays a role in down stream of Caspase 8 which is involved in Fas-mediated apoptosis.
  • Cellular localizationCytoplasmic

    • References for Caspase 1 protein (Active) (ab39901)

    ab39901 has not yet been referenced specifically in any publications.

    Publishing research using ab39901 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab39901 Submit a Question for ab39901

    Displaying 1 - 5 of 5 results for Abreviews and Q&A

    10
    Votes: Highest First

    I do understand what you are saying, but when production prepares the vials they MUST know how much that they are putting in. Is there a mass/vial or even an activity/mass associated with each lot that would allow us to figure this out. I am only being pe...

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    Thank you for your reply.

    I certainly understand that the mass concentration would be imperative when using in Western blot. Unfortunately this is considered proprietary information and I cannot provide it.
    I've looked through our catalog an...

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    How many micrograms are provided per vial? This productis the active caspase 1heterotetramer comprised of small subunits (10 kDa) and large subunits (20 kDa), correct?

    Thank you for your call earlier this week and for your patience while I have been in touch with the producing lab about ab39901.

    This protein is provided as the heterotetramer of the small (10 kDa) and large (20 kDa) subunits of caspase 1. It is...

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    Our lab want to purchase caspase 1 (ab39901) and caspase 3 (ab52101) for
    my project. We have one question regarding these products. We want to know
    if we are going to cut our protein (40ug) with caspase 1 or 3, how many ul of
    caspase 1...

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    Thank you for contacting Abcam.

    For ab39901, Caspase 1, we recommend using 1 unit/assay for analyzing caspase activity and this protein has a biological activity : >5000 units/mg and it is supplied lyophilized and it should be reconstituted t...

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    Dear Sir/Madame, I would like to know how you define one unit of enzymatic activity for the following product: Caspase 1 protein (Active) (ab39901)

    Thank you for your inquiry. Please see below for the unit definition for ab39901 (Caspase 1 protein (Active)): One unit of the recombinant Caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA (pNA: pnitroanaline) per hour...

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    in which step is the dilution buffer to be used? it is not mentioned in the protocol

    Thank you for contacting us. I have heard back from the lab with the following information: The dilution buffer can be used to dilute the caspase 1 protein (ab39901) when being used as a positive control. Thus, the protocol is correct as it is. Co...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"