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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease
Overview
Product name
Caspase 1 protein (Active)
See all Caspase 1 products (2) ...
Protein description
Active Recombinant Mouse Caspase-1
Expression host
E. coli
Properties
Biological activity
SPECIFIC ACTIVITY: 20000 units/mg UNIT DEFINITION: One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate Ac-YVAD-pNA (pNA: pnitroanaline) per hour at 37oC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT
Form
Lyophilised:
Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70oC. Avoid multiple freeze/thaw cycles as activity might decrease.
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 15% Glycerol, PBS
Concentration
Concentration information loading...
Additional notes
The lyophilized caspase-1 is stable for 1 year at –70oC.
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS: Use at an assay dependent dilution.
Protein info
Relevance
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase 1 is similar to the cell death gene CED3 of C. elegans and regulates multiple proinflammatory cytokines, including Interleukin 1b and interferon-gamma-inducing factor. Caspase 1 plays a role in down stream of Caspase 8 which is involved in Fas-mediated apoptosis.
Cellular localization
Cytoplasmic
References for Caspase 1 protein (Active) (ab52079)
ab52079 has not yet been referenced specifically in any publications.
Publishing research using ab52079? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"