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Product name
Anti-Caspase 10 antibody [25C2]
See all Caspase 10 products (10) ...
Description
Rat monoclonal [25C2] to Caspase 10
Specificity
The antibody may be used to detect the full length caspase 10(59 kDa), the p17 subunit of caspase 10, and possibly an additional band of approximately 35 kDa.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: VKTFLEALPQ ESWQNKHAGS NGNRATNGAP SLVSRGMQGA SANTLNSETS TKRAAVYRMN RNHRGLCVIV NNHSFTSLKD RQGTHKDAEI LSHVFQWLGF TVHIHNNVTK VEMEMVLQKQ KCNPAHADGD CFVFCILTHG RFGAVYSSDE ALIPIREIMS HFTALQCPRL AEKPKLFFIQ ACQGEEIQPS VSIEAD, corresponding to amino acids 220-415 of Human Caspase 10.
VKTFLEALPQ ESWQNKHAGS NGNRATNGAP SLVSRGM QGA SANTLNSETS TKRAAVYRMN RNHRGLCVIV NNH SFTSLKD RQGTHKDAEI LSHVFQWLGF TVHIHNNVTK VEMEMVLQKQ KCNPAHADGD CFVFCILTHG RFGAVYS SDE ALIPIREIMS HFTALQCPRL AEKPKLFFIQ ACQ GEEIQPS VSIEAD
Epitope
p17 subunit of human caspase 10.
Positive control
Whole extract of cultured human acute T cell leukemia Jurkat cells.
General notes
For continuous use, store at 2-8 °C for up to one month.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilution samples should be discarded if not used within 12 hours.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor.
Clonality
Monoclonal
Clone number
25C2
Isotype
IgG2a
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: Use at a concentration of 5 - 10 µg/ml.
ab10453 detects full length caspace 10 (59 kDa), the p17 subunit of caspase 10, and possibly an additional band of approximately 35 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-
-AMC and Asp-Glu-Val-Asp-
-AMC.
Isoform C is proteolytically inactive.
Tissue specificity
Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.
Involvement in disease
Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.
Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
Defects in CASP10 are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
Sequence similarities
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains.
Post-translational
modifications
Cleavage by granzyme B and autocatalytic activity generate the two active subunits.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Target information above from: UniProt accessionQ92851
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ALPS 2 antibody
- ALPS2 antibody
- Apoptosis related cysteine peptidase antibody
see all
Database links
- Entrez Gene: 843 Human
- Omim: 601762 Human
- SwissProt: Q92851 Human
- Unigene: 5353 Human
References for Anti-Caspase 10 antibody [25C2] (ab10453)
ab10453 has not yet been referenced specifically in any publications.
Publishing research using ab10453? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"