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Product name
Anti-Caspase 10 antibody
See all Caspase 10 products (10) ...
Description
Rabbit polyclonal to Caspase 10
Specificity
Anti-Caspase 10 specifically recognizes the proform and the active cleaved form of human caspase 10.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: GRFGAVYSSDEALI conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 359-372 of Human Caspase 10.
GRFGAVYSSDEALI
Epitope
Anti-Caspase 10 reacts with an epitope present in both caspase 10a (Mch4) and 10b (FLICE2).
Positive control
Whole extract of Jurkat human acute T leukemia cells extract.
General notes
For continuous use, store at 2-8 °C for up to one month.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilution samples should be discarded if not used within 12 hours.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500. Predicted molecular weight: 59 kDa.
Target
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-
-AMC and Asp-Glu-Val-Asp-
-AMC.
Isoform C is proteolytically inactive.
Tissue specificity
Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.
Involvement in disease
Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.
Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
Defects in CASP10 are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
Sequence similarities
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains.
Post-translational
modifications
Cleavage by granzyme B and autocatalytic activity generate the two active subunits.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Target information above from: UniProt accessionQ92851
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ALPS 2 antibody
- ALPS2 antibody
- Apoptosis related cysteine peptidase antibody
see all
Database links
- Entrez Gene: 843 Human
- Omim: 601762 Human
- SwissProt: Q92851 Human
- Unigene: 5353 Human
References for Anti-Caspase 10 antibody (ab10452)
ab10452 has not yet been referenced specifically in any publications.
Publishing research using ab10452? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"