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- Proteins and Peptides
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Product name
Anti-Caspase 10 antibody
See all Caspase 10 products (10) ...
Description
Rabbit polyclonal to Caspase 10
Specificity
This antibody only recognizes FLICE2 form of caspase-10.
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide, corresponding to amino acids 505-521 of Human Caspase 10.
Epitope
ISAQTPRPPMRRWSSVS
Positive control
HeLa whole cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
ICC/IF: 1/50. Fix with PFA.
Target
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-
-AMC and Asp-Glu-Val-Asp-
-AMC.
Isoform C is proteolytically inactive.
Tissue specificity
Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.
Involvement in disease
Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.
Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
Defects in CASP10 are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
Sequence similarities
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains.
Post-translational
modifications
Cleavage by granzyme B and autocatalytic activity generate the two active subunits.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Target information above from: UniProt accessionQ92851
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ALPS 2 antibody
- ALPS2 antibody
- Apoptosis related cysteine peptidase antibody
see all
Database links
- Entrez Gene: 843 Human
- Omim: 601762 Human
- SwissProt: Q92851 Human
- Unigene: 5353 Human
Anti-Caspase 10 antibody images:
Western blot - Caspase 10 antibody (ab2012)
All lanes : Anti-Caspase 10 antibody (ab2012) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : K562 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Predicted band size : 59 kDa
Observed band size : 59 kDa
Immunocytochemistry/ Immunofluorescence - Caspase 10 antibody (ab2012)
ab2012 at 1/50 staining cultured mouse hippocampal astrocytes by ICC/IF. The astrocytes were maintained in culture for 2 days in the presence of 10% serum, fixed with 4% PFA in PBS for 15 min at room temperature and permeabilized for 5 min in 0.25% Triton X-100 in PBS. The tissue was incuabated with the antibody for 2 hours at room temperature. A Cy3 ® conjugated donkey anti-rabbit IgG (H+L) was used as the secondary.
This image is courtesy of an anonymous Abreview
References for Anti-Caspase 10 antibody (ab2012)
This product has been referenced in:
- Pagnan G et al. The combined therapeutic effects of bortezomib and fenretinide on neuroblastoma cells involve endoplasmic reticulum stress response. Clin Cancer Res 15:1199-209 (2009). WB; Human.Read more (PubMed: 19228726) »
- Green KA et al. Caspase-mediated Cleavage of Insulin Receptor Substrate. J Biol Chem 279:25149-56 (2004).Read more (PubMed: 15069074) »
See all 2 publications for this product
Publishing research using ab2012? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"