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Product name
Anti-Caspase 12 antibody
See all Caspase 12 products (7) ...
Description
Rabbit polyclonal to Caspase 12
Tested applications
Cross reactivity
Reacts with
Mouse
Immunogen
Synthetic peptide: CTPSSPSESKRKVEDDE, corresponding to amino acids 100-116 of Mouse Caspase 12
CTPSSPSESKRKVEDDE
Positive control
Mouse brain tissue lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases
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Western blot - Caspase 12 antibody (ab34363)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab34363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 48 kDa).Can be blocked with Caspase 12 (N-term) peptide (mouse) (ab8374).
Target
Function
Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF.
Tissue specificity
Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis.
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain.
Target information above from: UniProt accessionQ6UXS9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptosis related cysteine protease antibody
- CASP 12 antibody
- CASP-12 antibody
see all
Database links
- Entrez Gene: 12364 Mouse
- SwissProt: O08736 Mouse
Anti-Caspase 12 antibody images:
Western blot - Caspase 12 antibody (ab34363)
All lanes : Anti-Caspase 12 antibody (ab34363) at 1 µg/ml
Lane 1 : murine brain tissue lysate 200 µg at 2mg/ml with no blocking peptide
Lane 2 : murine brain tissue lysate 200 µg at 2mg/ml with with blocking peptide 50 µg at 0.2mg/ml
Predicted band size : 48 kDa
Observed band size : 54 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Caspase 12 antibody (ab34363)
ab34363 has not yet been referenced specifically in any publications.
Publishing research using ab34363? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"