Recombinant
RabMAb

Anti-Caspase-14 antibody [EPR12927] (ab174847)

Overview

  • Product name
    Anti-Caspase-14 antibody [EPR12927]
    See all Caspase-14 primary antibodies
  • Description
    Rabbit monoclonal [EPR12927] to Caspase-14
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

  • Positive control
    • MCF7, ECV-304, HACAT and Human skin lysates; fetal skin tissue; Human skin tissue; pellet from fetal skin lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Caspase-14 antibody (Alexa Fluor® 488) [EPR12927] (ab200077)

    Anti-Caspase-14 antibody (HRP) [EPR12927] (ab200079)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR12927
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P 1/100 - 1/250.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/10 - 1/100.

Target

  • Function
    Believed to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin.
  • Tissue specificity
    Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels.
  • Sequence similarities
    Belongs to the peptidase C14A family.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis related cysteine protease antibody
    • CASP 14 antibody
    • CASP-14 antibody
    • CASP14 antibody
    • Caspase 14 apoptosis related cysteine protease antibody
    • Caspase 14 precursor antibody
    • Caspase-14 subunit p10 antibody
    • Caspase14 antibody
    • CASPE_HUMAN antibody
    • MGC119078 antibody
    • MGC119079 antibody
    • MICE antibody
    • Mini ICE antibody
    see all

Anti-Caspase-14 antibody [EPR12927] images

  • All lanes : Anti-Caspase-14 antibody [EPR12927] (ab174847) at 1/1000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : ECV-304 cell lysate
    Lane 3 : HACAT cell lysate
    Lane 4 : Human skin lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 28 kDa
  • Immunofluorescent staining of HaCaT cells labeling Caspase-14 (green) with ab174847 at 1:250 dilution, and labeling tubulin (red) with ab7291 at 1:500 (2 µg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) was used at 1:200 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) was used at 1:200.

    -ve control 1– Rabbit primary and anti-mouse secondary antibody. -ve control 2 – Mouse primary antibody and anti-rabbit secondary antibody.

  • Immunohistochemical analysis of paraffin-embedded fetal skin tissue labeling Caspase-14 using ab174847 at a 1:100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Caspase-14 using ab174847 at a 1:100 dilution.

  • Flow cytometric analysis of permeabilized HACAT cells labeling Caspase-14 using ab174847 at a 1:10 dilution (red), or rabbit IgG negative control (green).

  • Western blot analysis on immunoprecipitation pellet from fetal skin lysate (lane 1) or 1X PBS (lane 2, negative control) using ab174847 at 1:10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

References for Anti-Caspase-14 antibody [EPR12927] (ab174847)

ab174847 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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