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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Our Abpromise guarantee covers the use of ab174847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 28 kDa.|
|IHC-P||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.|
|IP||1/10 - 1/100.|
Immunofluorescent staining of HaCaT cells labeling Caspase-14 (green) with ab174847 at 1:250 dilution, and labeling tubulin (red) with ab7291 at 1:500 (2 µg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) was used at 1:200 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) was used at 1:200.
-ve control 1– Rabbit primary and anti-mouse secondary antibody. -ve control 2 – Mouse primary antibody and anti-rabbit secondary antibody.
Immunohistochemical analysis of paraffin-embedded fetal skin tissue labeling Caspase-14 using ab174847 at a 1:100 dilution.
Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Caspase-14 using ab174847 at a 1:100 dilution.
Flow cytometric analysis of permeabilized HACAT cells labeling Caspase-14 using ab174847 at a 1:10 dilution (red), or rabbit IgG negative control (green).
Western blot analysis on immunoprecipitation pellet from fetal skin lysate (lane 1) or 1X PBS (lane 2, negative control) using ab174847 at 1:10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab174847 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"