Overview

  • Product nameAnti-Caspase-14 antibodySee all Caspase-14 primary antibodies ...
  • Description
    Rabbit polyclonal to Caspase-14
  • SpecificityThis antibody is specific for Caspase-14.
  • Tested applicationsIHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-14 (C terminal).
    (Peptide available as ab72738)

  • Positive control
    • Jurkat cell lysate This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2 and HeLa.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab45415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2.5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).Can be blocked with Caspase-14 peptide (ab72738).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionBelieved to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin.
  • Tissue specificityExpressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels.
  • Sequence similaritiesBelongs to the peptidase C14A family.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis related cysteine protease antibody
    • Apoptosis related cysteine protease antibody
    • CASP 14 antibody
    • CASP-14 antibody
    • CASP14 antibody
    • Caspase 14 apoptosis related cysteine protease antibody
    • Caspase 14 apoptosis related cysteine protease antibody
    • Caspase 14 precursor antibody
    • Caspase 14 precursor antibody
    • Caspase-14 subunit p10 antibody
    • Caspase14 antibody
    • CASPE_HUMAN antibody
    • MGC119078 antibody
    • MGC119079 antibody
    • MICE antibody
    • MICE antibody
    • Mini ICE antibody
    • Mini ICE antibody
    see all

Anti-Caspase-14 antibody images

  • ab45415 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab45415 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5min) HeLa cells at 5ug/ml.

  • All lanes : Anti-Caspase-14 antibody (ab45415) at 1 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate + blocking peptide

    Secondary
    anti-rabbit IgG

    Predicted band size : 27 kDa
    Observed band size : 27 kDa

References for Anti-Caspase-14 antibody (ab45415)

This product has been referenced in:
  • Asselin-Labat ML  et al. Gata-3 negatively regulates the tumor-initiating capacity of mammary luminal progenitor cells and targets the putative tumor suppressor caspase-14. Mol Cell Biol 31:4609-22 (2011). WB . Read more (PubMed: 21930782) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Keratinocytes)
Loading amount 20 µg
Specification Keratinocytes
Gel Running Conditions Reduced Denaturing (4~12% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. HYOUNG JUNE KIM

Verified customer

Submitted Oct 26 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"