You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Caspase 2 Assay Kit (Fluorometric) (ab39794)

Submit a Question

Overview

  • Product nameCaspase 2 Assay Kit (Fluorometric)See all Caspase 2 kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay typeEnzyme activity
  • Assay time
    2h 00m
  • Product overview

    Abcam's Caspase 2 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase 2 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCaspase 2 has a positive and a negative role in the regulation of the apoptotic process. Overexpression of the full length Caspase 2 (Nedd2/Ich1 ) mRNA in some cell types results in apoptosis, whereas overexpression of an alternative Caspase 2 splice variant suppresses apoptosis induced by serum withdrawal. Caspase 2 may be activated in vitro by Caspase 1, Caspase 3 and the neutral serine protease granzyme B which is stored in the specialized lytic granules of cytotoxic lymphocytes.
  • Cellular localizationCytoplasmic
    • Alternative names
        Apoptosis related cysteine peptidaseCASP 2Casp 2L Pro
        CASP2Caspase 2 apoptosis related cysteine proteaseCaspase2Developmentally down regulated 2ICH 1ICH 1 proteaseICH 1LICH 1L/1SICH1ICH1 proteaseICH1LICH1L/1SNEDD 2NEDD2NEDD2 apoptosis regulatory geneNeural precursor cell expressedNeural precursor cell expressed developmentally down regulated 2
      see all

    Applications

    Our Abpromise guarantee covers the use of ab39794 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Protocols

    References for Caspase 2 Assay Kit (Fluorometric) (ab39794)

    ab39794 has not yet been referenced specifically in any publications.

    Publishing research using ab39794 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab39794 Submit a Question for ab39794

    There are currently no Abreviews or Questions for ab39794.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"