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Caspase 2 Fluorometric Assay Kit (ab39794)

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    Overview

    Product name

    Caspase 2 Fluorometric Assay Kit
    See all Caspase 2 products (3) ...

    Notes

    Caspase 2 Fluorometric Assay Kit provides as simple and convenient means for assaying the activity of caspase that recognise the sequence VDVAD.

    The Caspase 2 Fluorometric Assay kit is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (Wavelength max = 400 nm); upon cleavage of the substrate by caspase 2 or related caspases, free AFC emits a yellow-green fluorescence (Wavelenghth max = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase 2 activity.

    Properties

    Storage instructions

    Store kit at -20°C (Store Lysis Buffer and 2X Reaction Buffer at +4°C after opening)

    Components 1 kit
    2X Reaction Buffer4 x 2ml
    Cell Lysis Buffer1 x 100ml
    DTT1 x 400µl
    VDVAD-AFC1 x 500µl

    Research areas

    Kits/ Lysates/ Other >> Kits >> Apoptosis Kits >> Caspase Assays Kits >> Caspase 2 assay kits
    Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
    Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
    Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases

    Relevance

    Caspase 2 has a positive and a negative role in the regulation of the apoptotic process. Overexpression of the full length Caspase 2 (Nedd2/Ich1 ) mRNA in some cell types results in apoptosis, whereas overexpression of an alternative Caspase 2 splice variant suppresses apoptosis induced by serum withdrawal. Caspase 2 may be activated in vitro by Caspase 1, Caspase 3 and the neutral serine protease granzyme B which is stored in the specialized lytic granules of cytotoxic lymphocytes.

    Cellular localization

    Cytoplasmic

    Alternative names

    • Apoptosis related cysteine peptidase
    • CASP 2
    • Casp 2L Pro
    • CASP2
    • Caspase 2 apoptosis related cysteine protease
    • Caspase2
    • Developmentally down regulated 2
    • ICH 1
    • ICH 1 protease
    • ICH 1L
    • ICH 1L/1S
    • ICH1
    • ICH1 protease
    • ICH1L
    • ICH1L/1S
    • NEDD 2
    • NEDD2
    • NEDD2 apoptosis regulatory gene
    • Neural precursor cell expressed
    • Neural precursor cell expressed developmentally down regulated 2
    see all

    Protocols

  • Caspase 2 Fluorometrics Assay Kit

    • References for Caspase 2 Fluorometric Assay Kit (ab39794)

    ab39794 has not yet been referenced specifically in any publications.

    Publishing research using ab39794? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"