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Product Name
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Caspase 2 Fluorometric Assay Kit
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Product type
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Kits
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Description
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Caspase 2 Fluorometric Assay Kit
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Research areas
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Kits/ Lysates/ Other >> Kits >> Apoptosis Kits >> Caspase Assays Kits
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
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Components
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| Kit Components | Volume |
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| Cell Lysis Buffer | 100ml | | 2X Reaction Buffer | 4 X 2ml | | VDVAD-AFC(1mM) | 0.5ml | | DTT(1M) | 0.4ml |
Note: The kit provides approx. 100 assays.
For a detailed set of instructions please see the protocol.
For further information on the reagents please refer the assay protocol.
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Storage instructions
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Store kit at -20°C (Store Lysis Buffer and 2X Reaction Buffer at +4°C after opening)
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Form
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Liquid
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Notes
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Caspase 2 Fluorometric Assay Kit provides as simple and convenient means for assaying the activity of caspase that recognise the sequence VDVAD.
The Caspase 2 Fluorometric Assay kit is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (Wavelength max = 400 nm); upon cleavage of the substrate by caspase 2 or related caspases, free AFC emits a yellow-green fluorescence (Wavelenghth max = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase 2 activity.
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Material safety datasheet (MSDS) for this product:
Caspase 2 Fluorometric Assay Kit MSDS
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Relevance
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Caspase 2 has a positive and a negative role in the regulation of the apoptotic process. Overexpression of the full length Caspase 2 (Nedd2/Ich1 ) mRNA in some cell types results in apoptosis, whereas overexpression of an alternative Caspase 2 splice variant suppresses apoptosis induced by serum withdrawal. Caspase 2 may be activated in vitro by Caspase 1, Caspase 3 and the neutral serine protease granzyme B which is stored in the specialized lytic granules of cytotoxic lymphocytes.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Caspase 2 Fluorometric Assay Kit is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Caspase 2
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Caspase 2 Fluorometric Assay Kit - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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