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Caspase 2 (active) FITC Staining Kit (ab65612)

Q&A (1)

Overview

  • Product nameCaspase 2 (active) FITC Staining KitSee all Caspase 2 kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeEnzyme activity
  • Assay time
    2h 00m
  • Product overview

    Abcam's Caspase 2 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase 2 in living cells. The assay utilizes the caspase 2 inhibitor, VDVAD-FMK, conjugated to FITC (FITC-VDVAD-FMK) as a marker. FITC-VDVAD-FMK is cell permeable, non-toxic, and irreversibly binds to activated caspase 2 in apoptotic cells. The FITC label allows detection of activated caspase 2 in apoptotic cells directly by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Activation of caspases plays a central role in apoptosis.

  • Tested applicationsFluorescence Microscopy, Functional Studies, Flow Cyt more details

Properties

  • RelevanceCaspase 2 has a positive and a negative role in the regulation of the apoptotic process. Overexpression of the full length Caspase 2 (Nedd2/Ich1 ) mRNA in some cell types results in apoptosis, whereas overexpression of an alternative Caspase 2 splice variant suppresses apoptosis induced by serum withdrawal. Caspase 2 may be activated in vitro by Caspase 1, Caspase 3 and the neutral serine protease granzyme B which is stored in the specialized lytic granules of cytotoxic lymphocytes.
  • Cellular localizationCytoplasmic
    • Alternative names
        Apoptosis related cysteine peptidaseCASP 2Casp 2L Pro
        CASP2Caspase 2 apoptosis related cysteine proteaseCaspase2Developmental down regulated 2ICH 1ICH 1 proteaseICH 1LICH 1L/1SICH1ICH1 proteaseICH1LICH1L/1SNEDD 2NEDD2NEDD2 apoptosis regulatory geneNeural precursor cell expressedNeural precursor cell expressed developmentally down regulated 2
      see all

    Applications

    Our Abpromise guarantee covers the use of ab65612 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Fluorescence Microscopy Fluorescence Microscopy
    Functional Studies FuncS
    Flow Cyt Flow Cyt

    Protocols

    References for Caspase 2 (active) FITC Staining Kit (ab65612)

    ab65612 has not yet been referenced specifically in any publications.

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    Can these kits be used to stain adherent cells?

    The developer of the kit suggests trypsinizing the cells and then using the protocol for cells in suspension. If you do the assay this way, it would probably be best any apply the apoptosis-inducing treatment to the cells after trypsinization, to the cell...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"