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Product name
Anti-Caspase 2 antibody
See all Caspase 2 products (12) ...
Description
Rabbit polyclonal to Caspase 2
Specificity
ab25896 recognises Caspase-2.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide, consisting of 16 N-terminal amino acids of Human Caspase 2
Positive control
Ramos cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at a concentration of 2 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 40 and 50 kDa of long and short isoforms, respectively. Depending on cell lines or tissues used, other cleavage products may also be observed. Can be blocked with Caspase 2 peptide (ab39724).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
Tissue specificity
Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain.
Post-translational
modifications
The mature protease can process its own propeptide, but not that of other caspases.
Target information above from: UniProt accessionP42575
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptosis related cysteine peptidase antibody
- CASP 2 antibody
- Casp 2L Pro antibody
see all
Database links
- Entrez Gene: 835 Human
- Entrez Gene: 12366 Mouse
- Omim: 600639 Human
- SwissProt: P42575 Human
- SwissProt: P29594 Mouse
- Unigene: 368982 Human
- Unigene: 3921 Mouse
Anti-Caspase 2 antibody images:
Western blot - Caspase 2 antibody (ab25896)
Lane 1 : Anti-Caspase 2 antibody (ab25896) at 0.5 µg/ml
Lane 2 : Anti-Caspase 2 antibody (ab25896) at 1 µg/ml
Lane 3 : Anti-Caspase 2 antibody (ab25896) at 2 µg/ml
Lane 1 : Ramos cells
Lane 2 : Ramos cells
Lane 3 : Ramos cells
Observed band size : 40,50 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Caspase 2 antibody (ab25896)
ab25896 at 2µg/ml staining Caspase 2 in A-20 cells by ICC/IF
References for Anti-Caspase 2 antibody (ab25896)
ab25896 has not yet been referenced specifically in any publications.
Publishing research using ab25896? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"