Caspase 3 Assay Kit (Fluorometric) (ab39383)
- Product nameCaspase 3 Assay Kit (Fluorometric)See all Caspase 3 kits ...
- Tests100 x 1 assay
- Sample typeTissue Extracts, Cell Lysate
- Assay typeEnzyme activity
- Assay time2h 00m
- Product overview
Abcam's Caspase 3 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the DEVD-dependent caspase activity. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity.
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Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests 2X Reaction Buffer 4 x 2ml Cell Lysis Buffer 1 x 100ml DEVD AFC 1 x 500µl DTT 1 x 400µl
- ApopainApopain precursor CASP 3
- CASP3Caspase 3 apoptosis related cysteine proteaseCaspase 3 p12 subunitCaspase 3 p17 subunitCaspase3CPP 32CPP32CPP32BCysteine protease CPP32Human cysteine protease CPP32 isoform alpha mRNA complete cdsICE3LICEPARP cleavage proteaseSCA 1SCA1SREBP cleavage activity 1Yama protein
Our Abpromise guarantee covers the use of ab39383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Caspase 3 Assay Kit (Fluorometric) (ab39383)
This product has been referenced in:
- Lee YJ et al. Apoptotic cell instillation after bleomycin attenuates lung injury through hepatocyte growth factor induction. Eur Respir J 40:424-35 (2012). Read more (PubMed: 22441736) »