Western blot abreview for Anti-active + pro Caspase 3 antibody

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Abreviews
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Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Schmidtea mediterranea Tissue lysate - whole (whole planarians)
Specification
whole planarians
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1500

Secondary antibody

Name
Non-Abcam antibody was used: anti HRP goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Bands
Specific: around 35-16 kDa
Exposure
6 hour(s), 0 minute(s) and 0 second(s)
Username

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Submitted Sep 23 2013

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