Western blot abreview for Anti-active + pro Caspase 3 antibody

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Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Schmidtea mediterranea Tissue lysate - whole (whole planarians)
Specification whole planarians
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time 12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution 1/1500

Secondary antibody

Name Non-Abcam antibody was used: anti HRP goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/2000


Detection method ECL
Bands Specific: around 35-16 kDa
Exposure 6 hour(s), 0 minute(s) and 0 second(s)

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Submitted Sep 23 2013