Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-active + pro Caspase 3 antibody

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Fish Tissue sections (Ovary)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6,0
Permeabilization
Yes - Triton
Specification
Ovary
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/50

Secondary antibody

Secondary antibody
None used

Additional data

Additional Notes
Fish species: Astyanax altiparanae (Characidae: Teleostei), also known as "lambari-do-rabo-amarelo" or "tambiú" Fixative concentration: 4% Permeabilisation: 0,5% Biotinylated anti-rabbit, anti-mouse and anti-goat immunoglobulins in phosphate buffered saline (PBS) containing stabilizing protein and 0.015 mol/L sodium azide. It belongs to the kit Dako LSAB2 System-HRP [detection system]
Abcam response
Thank you for your review in this untested species. We value you time and feedback.
Username

Dr. Monica Cassel

Verified customer

Submitted Jul 29 2014

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