Application
Western blot
Loading amount
1000 cells
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Sample
Mouse Cell lysate - whole cell (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: jackson anti-rb
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 17 kDa
Exposure
5 minute(s) and 0 second(s)
