Western blot abreview for Anti-active + pro Caspase 3 antibody

Application Western blot
Loading amount 1000 cells
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Sample Mouse Cell lysate - whole cell (Brain)
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Incubation time 12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution 1/1000

Secondary antibody

Name Non-Abcam antibody was used: jackson anti-rb
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/10000


Detection method ECL
Bands Specific: 17 kDa
Exposure 5 minute(s) and 0 second(s)

Abcam user community

Verified customer

Submitted Sep 23 2014