Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-active + pro Caspase 3 antibody

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 35°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample Rat Tissue sections (Bone - Femur)
Specification Bone - Femur
Permeabilization No
Fixative Paraformaldehyde

Other product details

Incubation time 15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T
Dilution 1/50

Secondary antibody

Name Non-Abcam antibody was used: Goat anti-rabbit IgG, F(ab')2-AP (sc-3838)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Dilution 1/100

Mr. Helder Fonseca

Verified customer

Submitted Oct 06 2014