Western blot abreview for Anti-active + pro Caspase 3 antibody

Excellent
Abreviews
Application Western blot
Sample Rat Tissue lysate - whole (Heart)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 65 µg
Specification Heart
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time 13 hour(s) and 30 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBS-T
Dilution 1/1000

Secondary antibody

Dilution 1/2000
Name Abcam antibody:Goat Anti-Rabbit IgG H&L (HRP)

Detection

Detection method ECL
Exposure 6 minute(s) and 20 second(s)
Bands Specific: 17, 32 kDa
Positive control Samples
Username

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Verified customer

Submitted Oct 06 2015