Western blot abreview for Anti-active + pro Caspase 3 antibody

Application Western blot
Sample Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 20 µg
Specification Kidney
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Milk in TBST
Dilution 1/1000

Secondary antibody

Dilution 1/5000
Name Non-Abcam antibody was used: Jackson ImmunoResearch
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase


Detection method ECL
Exposure 30 minute(s) and 0 second(s)
Bands Specific: 25 kDa

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Submitted Oct 09 2015