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Caspase 3 protein (Active) (ab52071)

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Overview

  • Product nameCaspase 3 protein (Active)See all Caspase 3 proteins and peptides ...
  • Protein descriptionRecombinant full length protein (Mouse)
  • Expression hostE. coli

    • Properties

  • FormLyophilised:Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 5% Glycerol, 50mM Sodium chloride, 50mM HEPES, 0.1% Chaps, 10mM DTT, 10mM EDTA, pH 7.2
  • Concentration information loading...
  • Additional notesThe lyophilized caspase-3 is stable for 1 year at –70degC. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70degC. Avoid multiple freeze/thaw cycles as activity might decrease.
  • Research Areas

    • Applications

  • Application notesSPECIFIC ACTIVITY: 300,000 units/mg
    One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37degC.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        A830040C14RikApopainCASP 3
        CASP-3CASP3CASP3_HUMANCasp3aCaspase 3Caspase 3, apoptosis-related cysteine peptidaseCaspase 3, apoptosis-related cysteine proteaseCaspase 3, apoptosis-related cysteine protease aCaspase-3 subunit p12CC3CPP 32CPP-32CPP32CPP32BCysteine protease CPP32EC 3.4.22.56LICEMGC100890MGC93645mldyOTTHUMP00000165052OTTHUMP00000165053OTTHUMP00000165054OTTHUMP00000165055OTTHUMP00000224230OTTHUMP00000224231PARP cleavage proteaseProcaspase3Protein YamaSCA 1SCA-1SCA1SREBP cleavage activity 1YamaYama proteinzgc:100890
      see all
  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • Tissue specificityHighly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • Sequence similaritiesBelongs to the peptidase C14A family.
  • Post-translational
    modifications    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession P42574 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for Caspase 3 protein (Active) (ab52071)

    ab52071 has not yet been referenced specifically in any publications.

    Publishing research using ab52071 ? Please let us know so that we can cite the reference in this datasheet

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    Hi.

    I'm interested in use one of your products as a positive control of active Casp3 (17kDa) by western blot. But I have doubts that I can use it. The webpage says "full length protein", but also "active" and the active form o...

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    Thank you for contacting us.

    The "active" means this protein will act as an enzyme. In WB the protein will be observed at 25-30 kDa.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need a...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"