Caspase 4 Assay Kit (Fluorometric) (ab65658)
- Product nameCaspase 4 Assay Kit (Fluorometric)See all Caspase 4 kits ...
- Tests100 x 1 assay
- Sample typeTissue Extracts, Cell Lysate
- Assay typeEnzyme activity
- Assay time2h 00m
- Product overview
Abcam's Caspase 4 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on detection of cleavage of substrate LEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEVD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase 4 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.
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Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests 2X Reaction Buffer 4 x 2ml Cell Lysis Buffer 1 x 100ml DTT 1 x 400µl LEVD-AFC (1mM) 1 x 500µl
- Research Areas
Our Abpromise guarantee covers the use of ab65658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Caspase 4 Assay Kit (Fluorometric) (ab65658)
ab65658 has not yet been referenced specifically in any publications.