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Overview

  • Product nameCaspase 4 protein (Active)See all Caspase 4 proteins and peptides ...
  • Protein descriptionRecombinant full length protein (Human)
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activity5000 units/mg.
  • FormLyophilised:Reconstitute to 1 unit per µl in PBS containing 15% glycerol. Aliquot and store immediately at -80°C.
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 15% Glycerol, PBS
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab51994 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies
  • Application notesFuncS: Caspase activity assay.
    We recommend using 1 unit/assay for analyzing caspase activity.
    One unit is the enzyme activity that cleaves 1 nmol of the caspase substrate WEHD-p-nitroanaline per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Apoptosis related cysteine peptidaseApoptotic cysteine protease Mih1/TXCASP 4
        CASP-4CASP4CASP4_HUMANCaspase 4 apoptosis related cysteine peptidaseCaspase-4 subunit 2Caspase4ICE(rel) IIICE(rel)-IIICE(rel)IIICEREL IIICERELIIICH 2ICH 2 proteaseICH2Mih1/TXProtease ICH-2Protease TXTXTX protease
      see all
  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1.
  • Tissue specificityWidely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications    The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8.

    • Target information above from: UniProt accession P49662 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for Caspase 4 protein (Active) (ab51994)

    ab51994 has not yet been referenced specifically in any publications.

    Publishing research using ab51994 ? Please let us know so that we can cite the reference in this datasheet

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