Overview

  • Product nameCaspase 5 Assay Kit (Colorimetric)
    See all Caspase 5 kits
  • Tests
    100 x 1 assay
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay typeEnzyme activity
  • Assay time
    2h 00m
  • Product overview

    Abcam's Caspase 5 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence WEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate WEHD-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
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  • Notes

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceCaspase 5 belongs to the Ced3/ICE family of cysteine proteases. Caspase 5 exists as an inactive proenzyme which undergoes proteolytic processing at conserved aspartic residues, leading to p20 and p10 subunits that dimerize to form the active enzyme. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.
  • Alternative names
    • CASP 5
    • CASP5
    • Caspase 5 apoptosis related cysteine peptidase
    • Caspase5
    • ICE(rel) III
    • ICE(rel)III
    • ICEREL III
    • ICERELIII
    • ICH 3
    • ICH 3 protease
    • ICH3
    • MGC141966
    • TY protease
    see all

Applications

Our Abpromise guarantee covers the use of ab65661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Protocols

References for Caspase 5 Assay Kit (Colorimetric) (ab65661)

ab65661 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"