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Overview

  • Product nameCaspase 5 protein (Active)See all Caspase 5 proteins and peptides ...
  • Protein descriptionActive Recombinant Human Caspase-5
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activitySPECIFIC ACTIVITY: 5000 units/mg
  • FormLyophilised:Reconstitute to 1 unit per µl in PBS containing 15% glycerol. Lyophilized caspase-5 is stable for 1 year at –70oC. Following reconstitution, the enzyme should be aliquoted and immediately stored at –70oC.
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 15% Glycerol, PBS
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab52109 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    Functional Studies
  • Application notesFuncS: 1 unit/assay for analyzing caspase activity is recommended.
    One unit of the recombinant caspase-5 is the enzyme activity that cleaves 1 nmol of the caspase substrate WEHD-pNA (pNA: pnitroanaline) per hour at 37oC, in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
    SDS-PAGE: Use at an assay dependent dilution.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Apoptosis related cysteine proteaseCASP-5CASP5
        CASP5_HUMANCaspase-5 subunit p10ICE(rel)-IIIICERELIIIICH 3ICH 3 proteaseProtease ICH-3Protease TYTYTY protease
      see all
  • FunctionMediator of programmed cell death (apoptosis).
  • Tissue specificityExpressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

    • Target information above from: UniProt accession P51878 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for Caspase 5 protein (Active) (ab52109)

    ab52109 has not yet been referenced specifically in any publications.

    Publishing research using ab52109 ? Please let us know so that we can cite the reference in this datasheet

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