Overview

  • Product nameCaspase 6 Assay Kit (Colorimetric)See all Caspase 6 kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay typeEnzyme activity
  • Assay time
    2h 00m
  • Product overview

    Abcam's Caspase 6 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VEID. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate VEID-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
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  • Notes

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCaspase 6 is involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Caspase 3 and Caspase 6 are the major active caspases in apoptotic cells, and are activated in response to distinct apoptosis inducing stimuli and in all cell lines analyzed. Both Caspase 3 and 6 are present in apoptotic cells as multiple active species. Caspase 6 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei.
  • Cellular localizationCytoplasmic
  • Alternative names
    • Apoptosis Related Cysteine Protease
    • Apoptosis Related Cysteine Protease
    • Apoptotic cysteine protease MCH2
    • Apoptotic protease MCH 2
    • Apoptotic Protease MCH2
    • Casp 6
    • CASP6
    • Caspase 6 apoptosis related cysteine protease
    • Caspase 6 precursor
    • Caspase6
    • Human cysteine protease Mch2 isoform alpha
    • Mch 2
    • MCH2
    see all

Applications

Our Abpromise guarantee covers the use of ab39709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies Use at an assay dependent concentration.

Protocols

References for Caspase 6 Assay Kit (Colorimetric) (ab39709)

ab39709 has not yet been referenced specifically in any publications.

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