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Product Name
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Caspase 6 Fluorometric Assay kit
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Product type
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Kits
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Description
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Caspase 6 Fluorometric Assay kit
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Research areas
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Kits/ Lysates/ Other >> Kits >> Apoptosis Kits >> Caspase Assays Kits
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
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Components
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| Kit Components | Volume |
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| Cell Lysis Buffer | 100ml | | 2X Reaction Buffer | 4 X 2ml | | VEID-AFC(1mM) | 0.5ml | | DTT(1M) | 0.4ml |
Note: The kit provides approx. 100 assays.
Note: For more detailed information view specific protocol
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Storage instructions
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Store kit at -20°C (Store Lysis Buffer and 2X Reaction Buffer at +4°C after opening)
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Form
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Liquid
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Notes
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The Caspase 6 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VEID.
The Caspase 6 Fluorometric assay is based on detection of cleavage of substrate VEID-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VEID-AFC emits blue light (Wavelength max = 400 nm); upon cleavage of the substrate by caspases 6, free AFC emits a yellow-green fluorescence (wavelength max = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase 6 activity.
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Material safety datasheet (MSDS) for this product:
Caspase 6 Fluorometric Assay kit MSDS
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Relevance
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Caspase 6 is involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Caspase 3 and Caspase 6 are the major active caspases in apoptotic cells, and are activated in response to distinct apoptosis inducing stimuli and in all cell lines analyzed. Both Caspase 3 and 6 are present in apoptotic cells as multiple active species. Caspase 6 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Caspase 6 Fluorometric Assay kit is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Caspase 6
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Caspase 6 Fluorometric Assay kit - more information
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Customer reviews (feedback) regarding Caspase 6 Fluorometric Assay kit |
Customer FAQs regarding Caspase 6 Fluorometric Assay kit |
Protocols for Caspase 6 Fluorometric Assay kit |
Price and availability of products related to Caspase 6 Fluorometric Assay kit |
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Caspase 6 Fluorometric Assay kit - related products:
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Caspase 6 Fluorometric Assay kit - other tools:
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Contact Abcam with a Technical Enquiry about Caspase 6 Fluorometric Assay kit |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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