Caspase 7 Inhibitor Drug Detection Kit (ab102495)

Overview

  • Product nameCaspase 7 Inhibitor Drug Detection Kit
  • Detection methodFluorescent
  • Tests
    100 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Assay time
    1h 30m
  • Product overview

    Abcam's Caspase 7 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate DEVD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 7 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 7 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
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  • Notes

    Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.

  • Tested applicationsFunctional Studies more details
  • PlatformMicroplate

Properties

  • RelevanceCaspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
  • Alternative names
    • CASP7 inhibitor

Applications

Our Abpromise guarantee covers the use of ab102495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies Use at an assay dependent dilution.

The assay was tested useing approxamatly 1-5 x 10^6 cells.

For the inhibitor, the same concentration range as mentioned for the control inhibitor (1 ul of 2 mM solution) should be used.

Caspase 7 Inhibitor Drug Detection Kit images

  • Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using DEVD-AFC as caspase substrate and following Caspase 7 Inhibitor Drug Detection Kit (ab102495) protocol. Lanes:

    1.- Background Control: no Caspase 7, no z-VMAD-FMK

    2.- Positive Inhibition Control: no Caspase 7, +z-VAD-FMK

    3.- Positive Control: + Caspase 7, no z-VAD-FMK

    4.- Caspase 7 + 2.5µM z-VAD-FMK

    5.- Caspase 7 + 10µM z-VAD-FMK

    6.- Caspase 7 + 40µM z-VAD-FMK

Protocols

References for Caspase 7 Inhibitor Drug Detection Kit (ab102495)

ab102495 has not yet been referenced specifically in any publications.

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I consulted the lab to make sure I can give you the information needed. The information in the protocol about sample preparation is very brief.

Under step one in the protocol for the kit you wi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"