Caspase 7 Inhibitor Drug Detection Kit (ab102495)
- Product nameCaspase 7 Inhibitor Drug Detection Kit
- Tests100 x 1 assay
- Sample typeAdherent cells, Suspension cells
- Assay typeQuantitative
- Assay time1h 30m
- Product overview
Abcam's Caspase 7 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate DEVD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 7 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 7 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
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Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests 2X Reaction Buffer 1 x 10ml Active Caspase-7 1 x 100 units Caspase Inhibitor, Z-VAD-FMK 1 x 10µl Caspase Substrate DEVD-AFC 1 x 0.5ml DTT 1 x 100µl
- Research Areas
- CASP7 inhibitor
Our Abpromise guarantee covers the use of ab102495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The assay was tested useing approxamatly 1-5 x 10^6 cells.
For the inhibitor, the same concentration range as mentioned for the control inhibitor (1 ul of 2 mM solution) should be used.
References for Caspase 7 Inhibitor Drug Detection Kit (ab102495)
ab102495 has not yet been referenced specifically in any publications.