![Western blot - Caspase 7 antibody [25B881.1] (ab45025) image](#ab45025_1.bmp)
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Product name
Anti-Caspase 7 antibody [25B881.1]
See all Caspase 7 products (17) ...
Description
Mouse monoclonal [25B881.1] to Caspase 7
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein, active form (Human)
Positive control
Jurkat cell lysates
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
25B881.1
Isotype
IgG1
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
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Western blot - Caspase 7 antibody [25B881.1] (ab45025)
![Western blot - Caspase 7 antibody [25B881.1] (ab45025) image](/ps/datasheet/Images/45/ab45025/ab45025_1.bmp)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab45025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
Target
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Overexpression promotes programmed cell death.
Tissue specificity
Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
Sequence similarities
Belongs to the peptidase C14A family.
Post-translational
modifications
Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP55210
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptosis related cysteine peptidase antibody
- Apoptosis Related Cysteine Protease antibody
- Apoptotic protease Mch-3 antibody
see all
Database links
- Entrez Gene: 840 Human
- Omim: 601761 Human
- SwissProt: P55210 Human
- Unigene: 9216 Human
Anti-Caspase 7 antibody [25B881.1] images:
Western blot - Caspase 7 antibody [25B881.1] (ab45025)
Lane 1 : Anti-Caspase 7 antibody [25B881.1] (ab45025) at 2 µg/ml
Lane 2 : Anti-Caspase 7 antibody [25B881.1] (ab45025) at 0.5 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate
Predicted band size : 34 kDa
Observed band size : 34 kDa
This antibody detects only the 35 kDa Caspase 7 corresponding to pro−Caspase 7 in non−apoptotic Jurkat cells.
References for Anti-Caspase 7 antibody [25B881.1] (ab45025)
ab45025 has not yet been referenced specifically in any publications.
Publishing research using ab45025? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"