![Western blot - Caspase 7 antibody [E22] (ab32522) image](#ab32522wb.gif)
![Immunohistochemistry (Paraffin-embedded sections) - Caspase 7 antibody [E22] (ab32522) image](#ab32522ihc.jpg)
![Immunocytochemistry/ Immunofluorescence - Caspase 7 antibody [E22] (ab32522) image](#ab32522if.jpg)
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Product name
Anti-Caspase 7 antibody [E22]
See all Caspase 7 products (17) ...
Description
Rabbit monoclonal [E22] to Caspase 7
Specificity
The antibody should recognize both pro-form and p20 cleaved-form. The antibody does not cross-react with other Caspase family members.
Tested applications
WB, IHC-P, ICC/IF, IPmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues near N-terminus of human Caspase-7.
Positive control
Jurkat cell lysate, human skin cancer tissue and HeLa cells.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
E22
Isotype
IgG
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/100.
IHC-P: 1/50 - 1/100.
IP: 1/10.
WB: 1/1000. Detects a band of approximately 34 kDa.
Is unsuitable for FACS.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Overexpression promotes programmed cell death.
Tissue specificity
Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
Sequence similarities
Belongs to the peptidase C14A family.
Post-translational
modifications
Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP55210
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptosis related cysteine peptidase antibody
- Apoptosis Related Cysteine Protease antibody
- Apoptotic protease Mch-3 antibody
see all
Database links
- Entrez Gene: 840 Human
- Omim: 601761 Human
- SwissProt: P55210 Human
- Unigene: 9216 Human
Anti-Caspase 7 antibody [E22] images:
Western blot - Caspase 7 antibody [E22] (ab32522)
![Western blot - Caspase 7 antibody [E22] (ab32522)](/ps/datasheet/Images/32/ab32522/ab32522wb.gif)
Anti-Caspase 7 antibody [E22] (ab32522) at 1/1000 dilution + Jurkat cell lysate
Predicted band size : 34 kDa
Observed band size : 34 kDa
Immunohistochemistry (Paraffin-embedded sections) - Caspase 7 antibody [E22] (ab32522)
![Immunohistochemistry (Paraffin-embedded sections) - Caspase 7 antibody [E22] (ab32522)](/ps/datasheet/Images/32/ab32522/ab32522ihc.jpg)
Immunohistochemical analysis of paraffin embedded human skin cancer tissue using ab32522 at 1/50 dilution.
Immunocytochemistry/ Immunofluorescence - Caspase 7 antibody [E22] (ab32522)
![Immunocytochemistry/ Immunofluorescence - Caspase 7 antibody [E22] (ab32522)](/ps/datasheet/Images/32/ab32522/ab32522if.jpg)
Immunofluorescent staining of HeLa cells using ab32522 at 1/100 dilution.
References for Anti-Caspase 7 antibody [E22] (ab32522)
This product has been referenced in:
- Edelmann B et al. Caspase-8 and caspase-7 sequentially mediate proteolytic activation of acid sphingomyelinase in TNF-R1 receptosomes. EMBO J 30:379-94 (2011). WB, ICC/IF; Human.Read more (PubMed: 21157428) »
See 1 publication for this product
Publishing research using ab32522? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"