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Product name
Anti-Caspase 7 antibody
See all Caspase 7 products (17) ...
Description
Rabbit polyclonal to Caspase 7
Specificity
ab25900 recognises Caspase-7.
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide, consisting of 16 C-terminal amino acids of Human Caspase 7
Positive control
Jurkat cell lysate, human skeletal muscle cell
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 2 µg/ml.
WB: Use a concentration of 0.5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).
Target
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Overexpression promotes programmed cell death.
Tissue specificity
Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
Sequence similarities
Belongs to the peptidase C14A family.
Post-translational
modifications
Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP55210
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptosis related cysteine peptidase antibody
- Apoptosis Related Cysteine Protease antibody
- Apoptosis Related Cysteine Protease antibody
see all
Database links
- Entrez Gene: 840 Human
- Entrez Gene: 12369 Mouse
- Entrez Gene: 64026 Rat
- Omim: 601761 Human
- SwissProt: P55210 Human
- SwissProt: P97864 Mouse
- Unigene: 9216 Human
- Unigene: 35687 Mouse
see all
Anti-Caspase 7 antibody images:
Western blot - Caspase 7 antibody (ab25900)
Lane 1 : Anti-Caspase 7 antibody (ab25900) at 0.5 µg/ml
Lane 2 : Anti-Caspase 7 antibody (ab25900) at 1 µg/ml
Lane 3 : Anti-Caspase 7 antibody (ab25900) at 2 µg/ml
Lane 1 : Human skeletal
muscle cell lysate
Lane 2 : Human skeletal
muscle cell lysate
Lane 3 : Human skeletal
muscle cell lysate
Predicted band size : 34 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Caspase 7 antibody (ab25900)
Immunohistochemical staining of human skeletal muscle (paraffin embedded sections) using ab25900 anti Caspase 7 at 2 µg/ml.
References for Anti-Caspase 7 antibody (ab25900)
ab25900 has not yet been referenced specifically in any publications.
Publishing research using ab25900? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"