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Caspase 8 Assay Kit (Colorimetric) (ab39700)

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Overview

  • Product nameCaspase 8 Assay Kit (Colorimetric)See all Caspase 8 kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay typeEnzyme activity
  • Assay time
    2h 00m
  • Product overview

    Abcam's Caspase 8 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate IETD-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405 nm. Comparison of the absorbance of p-NA from a treated sample with an untreated control allows determination of the fold increase in Caspase 1 activity.
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  • Notes

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCaspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
  • Cellular localizationCytoplasmic
    • Alternative names
        Apoptosis related cysteine peptidaseApoptotic cysteine proteaseApoptotic protease Mch 5
        CAP 4CAP4CASP 8CASP8Caspase 8Caspase8CED 3FADD homologous ICE/CED 3 like proteaseFADD Homologous ICE/CED3 Like ProteaseFADD Like ICEFLICEICE like apoptotic protease 5MACHMACH5MCH 5MCH5MGC78473MORT1 associated CED 3 homologMORT1 associated CED3 homolog
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    Applications

    Our Abpromise guarantee covers the use of ab39700 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Protocols

    References for Caspase 8 Assay Kit (Colorimetric) (ab39700)

    ab39700 has not yet been referenced specifically in any publications.

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